Your browser doesn't support javascript.
loading
The histone chaperone SPT2 regulates chromatin structure and function in Metazoa.
Saredi, Giulia; Carelli, Francesco N; Rolland, Stéphane G M; Furlan, Giulia; Piquet, Sandra; Appert, Alex; Sanchez-Pulido, Luis; Price, Jonathan L; Alcon, Pablo; Lampersberger, Lisa; Déclais, Anne-Cécile; Ramakrishna, Navin B; Toth, Rachel; Macartney, Thomas; Alabert, Constance; Ponting, Chris P; Polo, Sophie E; Miska, Eric A; Gartner, Anton; Ahringer, Julie; Rouse, John.
Afiliación
  • Saredi G; MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee, UK. g.saredi@dundee.ac.uk.
  • Carelli FN; Gurdon Institute, University of Cambridge, Cambridge, UK.
  • Rolland SGM; Department of Genetics, University of Cambridge, Cambridge, UK.
  • Furlan G; IBS Centre for Genomic Integrity at Ulsan National Institute of Science and Technology (UNIST), Ulsan, Republic of Korea.
  • Piquet S; Gurdon Institute, University of Cambridge, Cambridge, UK.
  • Appert A; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Sanchez-Pulido L; Transine Therapeutics, Babraham Hall, Cambridge, UK.
  • Price JL; Laboratory of Epigenome Integrity, Epigenetics and Cell Fate Centre, UMR 7216 CNRS - Université Paris Cité, Paris, France.
  • Alcon P; Gurdon Institute, University of Cambridge, Cambridge, UK.
  • Lampersberger L; Department of Genetics, University of Cambridge, Cambridge, UK.
  • Déclais AC; MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, Edinburgh, UK.
  • Ramakrishna NB; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, UK.
  • Toth R; Gurdon Institute, University of Cambridge, Cambridge, UK.
  • Macartney T; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Alabert C; MRC Laboratory of Molecular Biology, Cambridge, UK.
  • Ponting CP; Gurdon Institute, University of Cambridge, Cambridge, UK.
  • Polo SE; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Miska EA; Maxion Therapeutics, Unity Campus, Cambridge, UK.
  • Gartner A; Molecular Cell and Developmental Biology Division, School of Life Sciences, University of Dundee, Dundee, UK.
  • Ahringer J; Gurdon Institute, University of Cambridge, Cambridge, UK.
  • Rouse J; Department of Biochemistry, University of Cambridge, Cambridge, UK.
Nat Struct Mol Biol ; 31(3): 523-535, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38238586
ABSTRACT
Histone chaperones control nucleosome density and chromatin structure. In yeast, the H3-H4 chaperone Spt2 controls histone deposition at active genes but its roles in metazoan chromatin structure and organismal physiology are not known. Here we identify the Caenorhabditis elegans ortholog of SPT2 (CeSPT-2) and show that its ability to bind histones H3-H4 is important for germline development and transgenerational epigenetic gene silencing, and that spt-2 null mutants display signatures of a global stress response. Genome-wide profiling showed that CeSPT-2 binds to a range of highly expressed genes, and we find that spt-2 mutants have increased chromatin accessibility at a subset of these loci. We also show that SPT2 influences chromatin structure and controls the levels of soluble and chromatin-bound H3.3 in human cells. Our work reveals roles for SPT2 in controlling chromatin structure and function in Metazoa.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Unión al ADN / Chaperonas de Histonas Límite: Animals / Humans Idioma: En Revista: Nat Struct Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Unión al ADN / Chaperonas de Histonas Límite: Animals / Humans Idioma: En Revista: Nat Struct Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos