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Evaluation of long-term preservation methods for viral RNA in mosquitoes at room temperature.
Kai, Izumi; Kobayashi, Daisuke; Itokawa, Kentaro; Sanjoba, Chizu; Itoyama, Kyo; Isawa, Haruhiko.
Afiliación
  • Kai I; Graduate school of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki-shi, Kanagawa, Japan; Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, Japan.
  • Kobayashi D; Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, Japan; Management Department of Biosafety, Laboratory Animal, and Pathogen Bank, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, Japan. Electronic address: dk
  • Itokawa K; Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, Japan.
  • Sanjoba C; Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
  • Itoyama K; Graduate school of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki-shi, Kanagawa, Japan.
  • Isawa H; Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, Japan.
J Virol Methods ; 325: 114887, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38237867
ABSTRACT
Mosquitoes are important vectors of various pathogenic viruses. Almost all viruses transmitted by mosquitoes are RNA viruses. Therefore, to detect viral genes, mosquito samples must be kept at low temperatures to prevent RNA degradation. However, prolonged transport from the field to laboratory can pose challenges for temperature control. The aim of this study was to evaluate methods for preserving viral RNA in mosquito bodies at room temperature. Virus-infected mosquito samples were immersed in ethanol, propylene glycol, and a commercially available nucleic acid preservation reagent at room temperature, and viral RNA stability was compared. As a result, for the two RNA viruses (San Gabriel mononegavirus and dengue virus 1) subjected to this experiment, no significant decrease in the viral RNA was observed for at least eight weeks after immersion in the reagents, and the amount of RNA remaining was equivalent to that of samples stored at - 80 °C. These results indicate that immersion storage in these reagents used in this study is effective in preserving viral RNA in mosquitoes under room temperature conditions and is expected to be implemented in epidemiologic surveillance that is not limited by the cold chain from the field to the laboratory.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aedes / Culicidae Límite: Animals Idioma: En Revista: J Virol Methods Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aedes / Culicidae Límite: Animals Idioma: En Revista: J Virol Methods Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos