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Synbiotic Combination between Lactobacillus paracasei VL8 and Mannan-Oligosaccharide Repairs the Intestinal Barrier in the Dextran Sulfate Sodium-Induced Colitis Model by Regulating the Intestinal Stem Cell Niche.
Zhang, Tuo; Cheng, Tiantian; Geng, Shuo; Mao, Kemin; Li, Xiyu; Gao, Jie; Han, Jun; Sang, Yaxin.
Afiliación
  • Zhang T; Department of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei CN 071000, China.
  • Cheng T; Department of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei CN 071000, China.
  • Geng S; Department of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei CN 071000, China.
  • Mao K; Department of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei CN 071000, China.
  • Li X; Department of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei CN 071000, China.
  • Gao J; Department of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei CN 071000, China.
  • Han J; Department of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei CN 071000, China.
  • Sang Y; Department of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei CN 071000, China.
J Agric Food Chem ; 72(4): 2214-2228, 2024 Jan 31.
Article en En | MEDLINE | ID: mdl-38237048
ABSTRACT
Previously, Lactobacillus paracasei VL8, a lactobacillus strain isolated from the traditional Finnish fermented dairy product Viili, demonstrated immunomodulatory and antibacterial effects. The prebiotic mannan-oligosaccharide (MOS) further promoted its antibacterial activity and growth performance, holding promise for maintaining intestinal health. However, this has not been verified in vivo. In this study, we elucidated the process by which L. paracasei VL8 and its synbiotc combination (SYN) with MOS repair the intestinal barrier function in dextran sodium sulfate (DSS)-induced colitis mice. SYN surpasses VL8 or MOS alone in restoring goblet cells and improving the tight junction structure. Omics analysis on gut microbiota reveals SYN's ability to restore Lactobacillus spp. abundance and promote tryptophan metabolism. SYN intervention also inhibits the DSS-induced hyperactivation of the Wnt/ß-catenin pathway. Tryptophan metabolites from Lactobacillus induce intestinal organoid differentiation. Co-housing experiments confirm microbiota transferability, replicating intestinal barrier repair. In conclusion, our study highlights the potential therapeutic efficacy of the synbiotic combination of Lactobacillus paracasei VL8 and MOS in restoring the damaged intestinal barrier and offers new insights into the complex crosstalk between the gut microbiota and intestinal stem cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis / Probióticos / Simbióticos / Lacticaseibacillus paracasei Límite: Animals Idioma: En Revista: J Agric Food Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis / Probióticos / Simbióticos / Lacticaseibacillus paracasei Límite: Animals Idioma: En Revista: J Agric Food Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos