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An Ion Concentration Polarization Microplatform for Efficient Enrichment and Analysis of ctDNA.
Wang, Zhiying; Liu, Ming; Lin, Haocheng; Zhu, Guiying; Dong, Zaizai; Wu, Nan; Fan, Yubo; Xu, Gaolian; Chang, Lingqian; Wang, Yang.
Afiliación
  • Wang Z; Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
  • Liu M; Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
  • Lin H; Department of Urology, Peking University Third Hospital, Beijing, 100191, China.
  • Zhu G; School of Biomedical Engineering/Med-X, Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
  • Dong Z; Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
  • Wu N; School of Engineering Medicine, Beihang University, Beijing 100083, China.
  • Fan Y; Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Thoracic Surgery II, Peking University Cancer Hospital & Institute, Beijing 100142, China.
  • Xu G; Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China.
  • Chang L; School of Engineering Medicine, Beihang University, Beijing 100083, China.
  • Wang Y; School of Biomedical Engineering/Med-X, Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
ACS Nano ; 18(4): 2872-2884, 2024 Jan 30.
Article en En | MEDLINE | ID: mdl-38236597
ABSTRACT
Strategies for rapid, effective nucleic acid processing hold tremendous significance to the clinical analysis of circulating tumor DNA (ctDNA), a family of important markers indicating tumorigenesis and metastasis. However, traditional techniques remain challenging to achieve efficient DNA enrichment, further bringing about complicated operation and limited detection sensitivity. Here, we developed an ion concentration polarization microplatform that enabled highly rapid, efficient enrichment and purification of ctDNA from a variety of clinical samples, including serum, urine, and feces. The platform demonstrated efficiently separating and enriching ctDNA within 30 s, with a 100-fold improvement over traditional methods. Integrating an on-chip isothermal amplification module, the platform further achieved 100-fold enhanced sensitivity in ctDNA detection, which significantly eliminated false-negative results in the serum or urine samples due to the low abundance of ctDNA. Such a simple-designed platform offers a user-friendly yet powerful diagnosis technique with a wide applicability, ranging from early tumor diagnosis to infection screening.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / ADN Tumoral Circulante / Neoplasias Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: ACS Nano Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / ADN Tumoral Circulante / Neoplasias Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: ACS Nano Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos