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Optical fiber surface plasmon resonance sensor using electroless-plated gold film for thrombin detection.
Huang, Yu; Pu, Xiaoyin; Qian, Husun; Chuang, Chin-Jung; Dong, Shanshan; Wu, Jiangling; Xue, Jianjiang; Cheng, Wei; Ding, Shijia; Li, Shengqiang.
Afiliación
  • Huang Y; Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China. huangyu@cigit.ac.cn.
  • Pu X; Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714, China. huangyu@cigit.ac.cn.
  • Qian H; Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China.
  • Chuang CJ; Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714, China.
  • Dong S; Department of Laboratory Medicine, The Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, Guizhou, China.
  • Wu J; Department of Opto-Electronic Engineering, National Dong Hwa University, Hualien, 97401, Taiwan, China.
  • Xue J; Department of Clinical Laboratory, Medical Sciences Research Center, University-Town Hospital of Chongqing Medical University, Chongqing, 401331, China.
  • Cheng W; Department of Clinical Laboratory, Medical Sciences Research Center, University-Town Hospital of Chongqing Medical University, Chongqing, 401331, China. wujiangling@hospital.cqmu.edu.cn.
  • Ding S; Department of Clinical Laboratory, Medical Sciences Research Center, University-Town Hospital of Chongqing Medical University, Chongqing, 401331, China.
  • Li S; The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Anal Bioanal Chem ; 416(6): 1469-1483, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38236393
ABSTRACT
This paper describes the simple and label-free detection of thrombin using optical fiber surface plasmon resonance (SPR) sensors based on gold films prepared by the cost-effective method of electroless plating. The plating conditions for simultaneously obtaining gold film on cylindrical core and end surfaces of an optical fiber suitable for measurement were optimized. The fabricated sensor exhibited a linear refractive index sensitivity of 2150 nm/RIU and 7.136 (a.u.)/RIU in the refractive index of 1.3329-1.3605 interrogated by resonance wavelength and amplitude methods respectively and a single wavelength monitoring method was proposed to investigate the sensing performance of this sensor. Polyadenine diblock and thiolated thrombin aptamers were immobilized on gold nanoparticles and gold films respectively to implement a sandwich optical fiber assay for thrombin. The developed optical fiber SPR sensors were successfully used in the determination of thrombin down to 0.56 nM over a wide range from 2 to 100 nM and showed good selectivity for thrombin, which indicated their potential clinical applications for biomedical samples.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanopartículas del Metal Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Bioanal Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanopartículas del Metal Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Bioanal Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania