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Dehydroandrographolide alleviates rheumatoid arthritis by inhibiting neutrophil activation via LMIR3 in collagen induced arthritis rats.
Kong, Ning; Guan, Huanshuai; Duan, Xudong; Cao, Ruomu; Li, Heng; Xing, Fangze; Du, Xueshan; Zheng, Yi; Zhang, Lei; Li, Yiyang; Liu, Zeyu; Tian, Run; Wang, Kunzheng; Che, Delu; Yang, Pei.
Afiliación
  • Kong N; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Guan H; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Duan X; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Cao R; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Li H; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Xing F; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Du X; Department of Dermatology, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Zheng Y; Department of Dermatology, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Zhang L; Department of Pharmacy, Shaanxi Provincial Hospital of Traditional Chinese Medicine, Xi'an, China.
  • Li Y; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Liu Z; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Tian R; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Wang K; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Che D; Department of Dermatology, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
  • Yang P; Department of Bone and Joint Surgery, Xi'an Jiaotong University Second Affiliated Hospital, Xi'an, China.
Cell Cycle ; 23(1): 1-14, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38234233
ABSTRACT
Rheumatoid arthritis (RA) is an inflammatory disease which causes severe pain and disability. Neutrophils play essential roles in the onset and progression of RA; thus, inhibition of neutrophil activation is becoming a popular therapeutic strategy. Dehydroandrographolide has provided satisfactory outcomes in inflammatory diseases; however, its therapeutic effects and mechanism in RA are not fully understood. Leukocyte mono-immunoglobulin-like receptor 3 (LMIR3) is a negative regulator highly expressed in neutrophils. To determine whether dehydroandrographolide negatively regulated neutrophils activation via LMIR3, cytokines release and collagen-induced arthritis (CIA) rats were used in vitro and in vivo. Biacore, molecular docking analysis and molecular dynamics simulation were performed to prove the target of dehydroandrographolide. Moreover, the downstream signaling pathways of LMIR3 activation were analyzed by western blotting. Results showed that oral dehydroandrographolide administration of 2 mg/kg/day to CIA rats attenuated synovitis and bone and cartilage damage after the 28-day intervention, revealed using HE sections and micro-CT. Dehydroandrographolide significantly inhibited cytokine release and chemotaxis of LPS/TNF-α-activated neutrophils in vitro. Dehydroandrographolide inhibited neutrophils activation via binding to LMIR3. Moreover, dehydroandrographolide up-regulated the phosphorylation of SHP-1 and SHP-2, which are the essential kinases in the LMIR3 signaling pathways. This study revealed that dehydroandrographolide attenuated collagen-induced arthritis by suppressing neutrophil activation via LMIR3.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Experimental / Artritis Reumatoide / Diterpenos Límite: Animals Idioma: En Revista: Cell Cycle Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Artritis Experimental / Artritis Reumatoide / Diterpenos Límite: Animals Idioma: En Revista: Cell Cycle Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos