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Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: not as effective as cells.
Zomer, Helena Debiazi; de Souza Lima, Victor Juan; Bion, Monique Coelho; Brito, Karynne Nazare Lins; Rode, Michele; Stimamiglio, Marco Augusto; Jeremias, Talita da Silva; Trentin, Andrea Gonçalves.
Afiliación
  • Zomer HD; Department of Physiological Sciences, University of Florida, Gainesville, USA. helenazomer@ufl.edu.
  • de Souza Lima VJ; Department of Cell Biology, Embryology, and Genetics, Federal University of Santa Catarina, Florianópolis, Brazil. helenazomer@ufl.edu.
  • Bion MC; Department of Cell Biology, Embryology, and Genetics, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Brito KNL; Department of Cell Biology, Embryology, and Genetics, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Rode M; National Institute of Translational Neuroscience, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Stimamiglio MA; Department of Cell Biology, Embryology, and Genetics, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Jeremias TDS; Department of Cell Biology, Embryology, and Genetics, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Trentin AG; Laboratory for Stem Cells Basic Biology, Carlos Chagas Institute, FIOCRUZ/PR, Curitiba, Paraná, Brazil.
Stem Cell Res Ther ; 15(1): 15, 2024 01 17.
Article en En | MEDLINE | ID: mdl-38229157
ABSTRACT

BACKGROUND:

Although the paracrine effects of mesenchymal stem/stromal cells (MSCs) have been recognized as crucial mediators of their regenerative effects on tissue repair, the potential of MSC secretomes as effective substitutes for cellular therapies remains underexplored.

METHODS:

In this study, we compared MSCs from the human dermis (DSCs) and adipose tissue (ASCs) with their secretomes regarding their efficacy for skin wound healing using a translationally relevant murine model.

RESULTS:

Proteomic analysis revealed that while there was a substantial overlap in protein composition between DSC and ASC secretomes, specific proteins associated with wound healing and angiogenesis were differentially expressed. Despite a similar angiogenic potential in vivo, DSC and ASC secretomes were found to be less effective than cells in accelerating wound closure and promoting tissue remodeling.

CONCLUSIONS:

Overall, secretome-treated groups showed intermediary results between cells- and control-treated (empty scaffold) groups. These findings highlight that although secretomes possess therapeutic potential, their efficacy might be limited compared to cellular therapies. This study contributes to the growing understanding of MSC secretomes, emphasizes the need for further protocol optimization, and offers insights into their potential applications in regenerative medicine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Mesenquimatosas / Secretoma Tipo de estudio: Guideline / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Stem Cell Res Ther Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Mesenquimatosas / Secretoma Tipo de estudio: Guideline / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Stem Cell Res Ther Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido