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Investigation of the effects of Toxoplasma gondii on behavioral and molecular mechanism in bradyzoite stage.
Akcay, Guven; Bahadir, Anzel; Tatar, Yakup; Nuri Atalar, Mehmet; Babur, Cahit; Taylan Ozkan, Aysegul.
Afiliación
  • Akcay G; Hitit University, Faculty of Medicine, Department of Biophysics, Çorum, Turkey. Electronic address: guvenakcay@hitit.edu.tr.
  • Bahadir A; Duzce University, Faculty of Medicine, Department of Biophysics, Düzce, Turkey.
  • Tatar Y; TOBB University of Economics and Technology, Faculty of Medicine, Department of Physiology, Ankara, Turkey.
  • Nuri Atalar M; Igdir University, Faculty of Health Sciences, Department of Nutrition and Dietetics, Igdir, Turkey.
  • Babur C; National Parasitology Reference Laboratory, General Directorate of Health, Ministry of Health of Turkey, Ankara, Turkey.
  • Taylan Ozkan A; TOBB ETU University, Faculty of Medicine, Department of Medical Microbiology, Ankara, Turkey.
Brain Res ; 1828: 148762, 2024 Apr 01.
Article en En | MEDLINE | ID: mdl-38228258
ABSTRACT
Toxoplasma gondii is a single-celled parasite that causes a disease called toxoplasmosis. It can reach the central nervous system, but the mechanism of T. gondii disrupting the functioning of these brain regions occurs in bradyzoite stage of parasite, causing brain damage by forming tissue cysts in brain. In our study, the effects of T. gondii on locomotor activity, anxiety, learning and memory, and norepinephrine (NE), levodopa (L-DOPA), dopamine (DA) and 3,4-D-dihydroxyphenylacetic acid (DOPAC) catecholamines in amygdala, striatum, prefrontal cortex and hippocampus regions of the brain were investigated in bradyzoite stage. Twenty male Albino mice Mus musculus, 4-5 weeks old, weighing 20-25 g, were used. T. gondii inoculated to mice intraperitonealy with 48-50-hour passages of T. gondii RH Ankara strain. For intraperitoneal inoculation of mice 5x104 tachyzoites per mouse. No inoculation was made in control group (n 20). Locomotor activity behavior in open field test (OFT), anxious behavior in elevated plus maze (EPM), and learning behavior in novel object recognition (NOR) tests were evaluated. NE, L-DOPA, DA and DOPAC were measured by HPLC in brain tissues of amygdala, striatum, prefrontal cortex and hippocampus. A decrease was observed in the locomotor activity, anxiety and learning values of the T. gondii group compared to the control group (p < 0.05). The heighten in NE and L-DOPA levels in amygdala tissue of T. gondii group compared to control group, an elevation in NE, L-DOPA, DA and DOPAC levels in striatum tissue, and an increase in levels of NE in prefrontal cortex tissue were detected in monoamine results. In hippocampus tissue, an increase was observed in DA levels, while a decrease was observed in NE, L-DOPA and DOPAC levels. In our study, it has been shown that T. gondii in bradyzoite stage reduces locomotor activity, causes learning and memory impairment, and has anxiogenic effects.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Toxoplasmosis Límite: Animals Idioma: En Revista: Brain Res Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Toxoplasma / Toxoplasmosis Límite: Animals Idioma: En Revista: Brain Res Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos