Your browser doesn't support javascript.
loading
Neuropeptide W facilitates chronic gastric ulcer healing by the regulation of cyclooxygenase and NF-κB signaling pathways.
Arabaci Tamer, Sevil; Mermer, Kadriye Sezen; Erdogan, Ömer; Çevik, Özge; Ercan, Feriha; Bagci, Cahit; Yegen, Berrak Ç.
Afiliación
  • Arabaci Tamer S; Department of Physiology, Sakarya University School of Medicine, Sakarya, Turkey.
  • Mermer KS; Department of Physiology, Marmara University School of Medicine, Istanbul, Turkey.
  • Erdogan Ö; Faculty of Medicine, Department of Biochemistry, Aydin Adnan Menderes University, Aydin, Turkey.
  • Çevik Ö; Faculty of Medicine, Department of Biochemistry, Aydin Adnan Menderes University, Aydin, Turkey.
  • Ercan F; Department of Histology & Embryology, Marmara University School of Medicine, Istanbul, Turkey.
  • Bagci C; Department of Physiology, Sakarya University School of Medicine, Sakarya, Turkey.
  • Yegen BÇ; Department of Physiology, Marmara University School of Medicine, Istanbul, Turkey. byegen@marmara.edu.tr.
Inflammopharmacology ; 32(2): 1519-1529, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38227096
ABSTRACT

AIMS:

Putative beneficial effects of neuropeptide W (NPW) in the early phase of gastric ulcer healing process and the involvement of cyclooxygenase (COX) enzymes were investigated in an acetic acid-induced gastric ulcer model. MAIN

METHODS:

In anesthetized male Sprague-Dawley rats, acetic acid was applied surgically on the serosa and then a COX-inhibitor (COX-2-selective NS-398, COX-1-selective ketorolac, or non-selective indomethacin; 2 mg/kg/day, 3 mg/kg/day or 5 mg/kg/day; respectively) or saline was injected intraperitoneally. One h after ulcer induction, omeprazole (20 mg/kg/day), NPW (0.1 µg/kg/day) or saline was intraperitoneally administered. Injections of NPW, COX-inhibitors, omeprazole or saline were continued for the following 2 days until rats were decapitated at the end of the third day. KEY

FINDINGS:

NPW treatment depressed gastric prostaglandin (PG) I2 level, but not PGE2 level. Similar to omeprazole, NPW treatment significantly reduced gastric and serum tumor necrosis factor-alpha and interleukin-1 beta levels and depressed the upregulation of nuclear factor kappa B (NF-κB) and COX-2 expressions due to ulcer. In parallel with the histopathological findings, treatment with NPW suppressed ulcer-induced increases in myeloperoxidase activity and malondialdehyde level and replenished glutathione level. However, the inhibitory effect of NPW on myeloperoxidase activity and NPW-induced increase in glutathione were not observed in the presence of COX-1 inhibitor ketorolac or the non-selective COX-inhibitor indomethacin.

SIGNIFICANCE:

In conclusion, NPW facilitated the healing of gastric injury in rats via the inhibition of pro-inflammatory cytokine production, oxidative stress and neutrophil infiltration as well as the downregulation of COX-2 protein and NF-κB gene expressions.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Úlcera Gástrica / Neuropéptidos / Transducción de Señal Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Inflammopharmacology Asunto de la revista: FARMACOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Úlcera Gástrica / Neuropéptidos / Transducción de Señal Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Inflammopharmacology Asunto de la revista: FARMACOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Suiza