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Comparison of neutralization potency across passive immunotherapy approaches as potential treatments for emerging infectious diseases.
Ranjbaran, Hossein; Ehteshaminia, Yahya; Nadernezhad, Mohammadreza; Jalali, Seyedeh Farzaneh; Jadidi-Niaragh, Farhad; Pagheh, Abdol Sattar; Enderami, Seyed Ehsan; Kenari, Saeid Abedian; Hassannia, Hadi.
Afiliación
  • Ranjbaran H; Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Ehteshaminia Y; Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Nadernezhad M; Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Jalali SF; Department of Hematology, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran.
  • Jadidi-Niaragh F; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Pagheh AS; Infectious Diseases Research Center, Birjand University of Medical Science, Birjand, Iran.
  • Enderami SE; Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Kenari SA; Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Hassannia H; Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Heliyon ; 10(1): e23478, 2024 Jan 15.
Article en En | MEDLINE | ID: mdl-38226283
ABSTRACT
The use of passive immunotherapy, either as plasma or purified antibodies, has been recommended to treat the emerging infectious diseases (EIDs) in the absence of alternative therapeutic options. Here, we compare the neutralization potency of various passive immunotherapy approaches designed to provide the immediate neutralizing antibodies as potential EID treatments. To prepare human plasma and purified IgG, we screened and classified individuals into healthy, convalescent, and vaccinated groups against SARS-CoV-2 using qRT-PCR, anti-nucleocapsid, and anti-spike tests. Moreover, we prepared purified IgG from non-immunized and hyperimmunized rabbits against SARS-CoV-2 spike protein. Human and rabbit samples were used to evaluate the neutralization potency by sVNT. All vaccinated and convalescent human plasma and purified IgG groups, as well as purified IgG from hyperimmunized rabbits, had significantly greater levels of spike-specific antibodies than the control groups. Furthermore, when compared to the other groups, the purified IgG from hyperimmunized rabbits exhibited superior levels of neutralizing antibodies, with an IC50 value of 2.08 µg/ml. Additionally, our results indicated a statistically significant positive correlation between the neutralization IC50 value and the positive endpoint concentration of spike-specific antibodies. In conclusion, our study revealed that purified IgG from hyperimmunized animals has greater neutralization potency than other passive immunotherapy methods and may be the most suitable treatment of critically ill patients in EIDs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido