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Identification a novel Ganoderma FIP gene from Ganoderma capense and its functional expression in Pichia pastoris.
Lin, Jingwei; Liao, Yating; Yang, Sijia; Jin, Taicheng; Yu, Boning; Zhao, Kai; Sai, Yixiao; Lin, Cheng; Song, Yanhua; Ma, Hui; Wang, Zhanyong.
Afiliación
  • Lin J; College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
  • Liao Y; Liaoning Province Key Laboratory of Agricultural Technology, Shenyang, China.
  • Yang S; College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
  • Jin T; Liaoning Province Key Laboratory of Agricultural Technology, Shenyang, China.
  • Yu B; College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
  • Zhao K; Liaoning Province Key Laboratory of Agricultural Technology, Shenyang, China.
  • Sai Y; School of Life Science, Jilin Normal University, Siping, 136000, China.
  • Lin C; College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
  • Song Y; Liaoning Province Key Laboratory of Agricultural Technology, Shenyang, China.
  • Ma H; College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.
  • Wang Z; Liaoning Province Key Laboratory of Agricultural Technology, Shenyang, China.
World J Microbiol Biotechnol ; 40(2): 69, 2024 Jan 16.
Article en En | MEDLINE | ID: mdl-38225505
ABSTRACT
Ganoderma capense is a precious medicinal fungus in China. In this study, a novel fungal immunomodulatory protein gene, named as FIP-gca, was cloned from G. capense by homologous cloning. Sequencing analysis indicated that FIP-gca was composed of 336 bp, which encoded a polypeptide of 110 amino acids. Protein sequence blasting and phylogenetic analysis showed that FIP-gca shared homology with other Ganoderma FIPs. FIP-gca was effectively expressed in Pichia pastoris GS115 at an expression level of 166.8 mg/L and purified using HisTrap™ fast-flow prepack columns. The immunomodulation capacity of rFIP-gca was demonstrated by that rFIP-gca could obviously stimulate cell proliferation and increase IL-2 secretion of murine spleen lymphocytes. Besides, antitumor activity of rFIP-gca towards human stomach cancer AGS cell line was evaluated in vitro. Cell wound scratch assay proved that rFIP-gca could inhibit migration of AGS cells. And flow cytometry assay revealed that rFIP-gca could significantly induce apoptosis of AGS cells. rFIP-gca was able to induce 18.12% and 22.29% cell apoptosis at 0.3 µM and 0.6 µM, respectively. Conclusively, the novel FIP-gca gene from G. capense has been functionally expressed in Pichia and rFIP-gca exhibited ideal immunomodulation and anti-tumour activities, which implies its potential application and study in future.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ganoderma / Saccharomycetales Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: World J Microbiol Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ganoderma / Saccharomycetales Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: World J Microbiol Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania