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scRNA-seq analysis of cells comprising the amphioxus notochord.
Takahashi, Hiroki; Hisata, Kanako; Iguchi, Rin; Kikuchi, Sakura; Ogasawara, Michio; Satoh, Noriyuki.
Afiliación
  • Takahashi H; Interdisciplinary Research Unit, National Institute for Basic Biology, Okazaki, Aichi, 444-8585, Japan. Electronic address: taka@nibb.ac.jp.
  • Hisata K; Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa, 904-0495, Japan.
  • Iguchi R; Department of Biology, Graduate School of Science, Chiba University, Chiba, 262-8522, Japan.
  • Kikuchi S; Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa, 904-0495, Japan.
  • Ogasawara M; Department of Biology, Graduate School of Science, Chiba University, Chiba, 262-8522, Japan. Electronic address: ogasawara@faculty.chiba-u.jp.
  • Satoh N; Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa, 904-0495, Japan. Electronic address: norisky@oist.jp.
Dev Biol ; 508: 24-37, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38224933
ABSTRACT
Cephalochordates occupy a key phylogenetic position for deciphering the origin and evolution of chordates, since they diverged earlier than urochordates and vertebrates. The notochord is the most prominent feature of chordates. The amphioxus notochord features coin-shaped cells bearing myofibrils. Notochord-derived hedgehog signaling contributes to patterning of the dorsal nerve cord, as in vertebrates. However, properties of constituent notochord cells remain unknown at the single-cell level. We examined these properties using Iso-seq analysis, single-cell RNA-seq analysis, and in situ hybridization (ISH). Gene expression profiles broadly categorize notochordal cells into myofibrillar cells and non-myofibrillar cells. Myofibrillar cells occupy most of the central portion of the notochord, and some cells extend the notochordal horn to both sides of the ventral nerve cord. Some notochord myofibrillar genes are not expressed in myotomes, suggesting an occurrence of myofibrillar genes that are preferentially expressed in notochord. On the other hand, non-myofibrillar cells contain dorsal, lateral, and ventral Müller cells, and all three express both hedgehog and Brachyury. This was confirmed by ISH, although expression of hedgehog in ventral Müller cells was minimal. In addition, dorsal Müller cells express neural transmission-related genes, suggesting an interaction with nerve cord. Lateral Müller cells express hedgehog and other signaling-related genes, suggesting an interaction with myotomes positioned lateral to the notochord. Ventral Müller cells also expressed genes for FGF- and EGF-related signaling, which may be associated with development of endoderm, ventral to the notochord. Lateral Müller cells were intermediate between dorsal/ventral Müller cells. Since vertebrate notochord contributes to patterning and differentiation of ectoderm (nerve cord), mesoderm (somite), and endoderm, this investigation provides evidence that an ancestral or original form of vertebrate notochord is present in extant cephalochordates.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anfioxos Límite: Animals Idioma: En Revista: Dev Biol Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anfioxos Límite: Animals Idioma: En Revista: Dev Biol Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos