Your browser doesn't support javascript.
loading
Dynamics of macrophage polarization support Salmonella persistence in a whole living organism.
Leiba, Jade; Sipka, Tamara; Begon-Pescia, Christina; Bernardello, Matteo; Tairi, Sofiane; Bossi, Lionello; Gonzalez, Anne-Alicia; Mialhe, Xavier; Gualda, Emilio J; Loza-Alvarez, Pablo; Blanc-Potard, Anne; Lutfalla, Georges; Nguyen-Chi, Mai E.
Afiliación
  • Leiba J; LPHI, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Sipka T; LPHI, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Begon-Pescia C; LPHI, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Bernardello M; ICFO - Institute of Photonic Sciences, The Barcelona Institute of Science and Technology, Castelldefels, Barcelona, Spain.
  • Tairi S; LPHI, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Bossi L; Institute for Integrative Biology of the Cell-I2BC, Université Paris-Saclay, CEA, CNRS, Gif-sur-Yvette, France.
  • Gonzalez AA; MGX-Montpellier GenomiX, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Mialhe X; MGX-Montpellier GenomiX, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Gualda EJ; ICFO - Institute of Photonic Sciences, The Barcelona Institute of Science and Technology, Castelldefels, Barcelona, Spain.
  • Loza-Alvarez P; ICFO - Institute of Photonic Sciences, The Barcelona Institute of Science and Technology, Castelldefels, Barcelona, Spain.
  • Blanc-Potard A; LPHI, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Lutfalla G; LPHI, Université de Montpellier, CNRS, INSERM, Montpellier, France.
  • Nguyen-Chi ME; LPHI, Université de Montpellier, CNRS, INSERM, Montpellier, France.
Elife ; 132024 Jan 15.
Article en En | MEDLINE | ID: mdl-38224094
ABSTRACT
Numerous intracellular bacterial pathogens interfere with macrophage function, including macrophage polarization, to establish a niche and persist. However, the spatiotemporal dynamics of macrophage polarization during infection within host remain to be investigated. Here, we implement a model of persistent Salmonella Typhimurium infection in zebrafish, which allows visualization of polarized macrophages and bacteria in real time at high resolution. While macrophages polarize toward M1-like phenotype to control early infection, during later stages, Salmonella persists inside non-inflammatory clustered macrophages. Transcriptomic profiling of macrophages showed a highly dynamic signature during infection characterized by a switch from pro-inflammatory to anti-inflammatory/pro-regenerative status and revealed a shift in adhesion program. In agreement with this specific adhesion signature, macrophage trajectory tracking identifies motionless macrophages as a permissive niche for persistent Salmonella. Our results demonstrate that zebrafish model provides a unique platform to explore, in a whole organism, the versatile nature of macrophage functional programs during bacterial acute and persistent infections.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Interacciones Huésped-Patógeno Límite: Animals Idioma: En Revista: Elife Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Interacciones Huésped-Patógeno Límite: Animals Idioma: En Revista: Elife Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido