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Genome-wide identification of NAC transcription factors and regulation of monoterpenoid indole alkaloid biosynthesis in Catharanthus roseus.
Ahmed, Jawad; Sajjad, Yasar; Gatasheh, Mansour K; Ibrahim, Khalid Elfaki; Huzafa, Muhammad; Khan, Sabaz Ali; Situ, Chen; Abbasi, Arshad Mehmood; Hassan, Amjad.
Afiliación
  • Ahmed J; Department of Biotechnology, COMSATS University Islamabad, Abbottabad, Pakistan.
  • Sajjad Y; Institute for Global Food Security, School of Biological Sciences, Queens University Belfast, Belfast, United Kingdom.
  • Gatasheh MK; Department of Biotechnology, COMSATS University Islamabad, Abbottabad, Pakistan.
  • Ibrahim KE; Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
  • Huzafa M; Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
  • Khan SA; Department of Plant Sciences, Quaid-e-Azam University, Islamabad, Pakistan, Pakistan.
  • Situ C; Department of Biotechnology, COMSATS University Islamabad, Abbottabad, Pakistan.
  • Abbasi AM; Institute for Global Food Security, School of Biological Sciences, Queens University Belfast, Belfast, United Kingdom.
  • Hassan A; Department of Environmental Sciences, COMSATS University, Islamabad, Abbottabad, Pakistan.
Front Plant Sci ; 14: 1286584, 2023.
Article en En | MEDLINE | ID: mdl-38223288
ABSTRACT
NAC transcription factors (TFs) are crucial to growth and defense responses in plants. Though NACs have been characterized for their role in several plants, comprehensive information regarding their role in Catharanthus roseus, a perennial ornamental plant, is lacking. Homology modelling was employed to identify and characterize NACs in C. roseus. In-vitro propagation of C. roseus plants was carried out using cell suspension and nodal culture and were elicited with two auxin-antagonists, 5-fluoro Indole Acetic Acid (5-F-IAA) and α-(phenyl ethyl-2-oxo)-Indole-Acetic-Acid (PEO-IAA) for the enhanced production of monoterpenoid indole alkaloids (MIAs) namely catharanthine, vindoline, and vinblastine. Analyses revealed the presence of 47 putative CrNAC genes in the C. roseus genome, primarily localized in the nucleus. Phylogenetic analysis categorized these CrNACs into eight clusters, demonstrating the highest synteny with corresponding genes in Camptotheca acuminata. Additionally, at least one defense or hormone-responsive cis-acting element was identified in the promoter region of all the putative CrNACs. Of the two elicitors, 5-F-IAA was effective at 200 µM to elicit a 3.07-fold increase in catharanthine, 2.76-fold in vindoline, and 2.4-fold in vinblastine production in nodal culture. While a relatively lower increase in MIAs was recorded in suspension culture. Validation of RNA-Seq by qRT-PCR showed upregulated expression of stress-related genes (CrNAC-07 and CrNAC-24), and downregulated expression of growth-related gene (CrNAC-25) in elicited nodal culture of C. roseus. Additionally, the expression of genes involved in the biosynthesis of MIAs was significantly upregulated upon elicitation. The current study provides the first report on the role of CrNACs in regulating the biosynthesis of MIAs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Front Plant Sci Año: 2023 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Front Plant Sci Año: 2023 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Suiza