Suppression of photorespiratory metabolism by low O2 and presence of aminooxyacetic acid induces oxidative stress in Arabidopsis thaliana leaves.
Physiol Mol Biol Plants
; 29(12): 1851-1861, 2023 Dec.
Article
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| MEDLINE
| ID: mdl-38222271
ABSTRACT
Photorespiration, an essential component of plant metabolism, was upregulated under abiotic stress conditions, such as high light or drought. One of the signals for such upregulation was the rise in reactive oxygen species (ROS). Photorespiration was expected to mitigate oxidative stress by reducing ROS levels. However, it was unclear if ROS levels would increase when photorespiration was lowered. Our goal was to examine the redox status in leaves when photorespiratory metabolism was restricted under low O2 (medium flushed with N2 gas) or by adding aminooxyacetic acid (AOA), a photorespiratory inhibitor. We examined the impact of low O2 and AOA in leaves of Arabidopsis thaliana under dark, moderate, or high light. Downregulation of typical photorespiratory enzymes, including catalase (CAT), glycolate oxidase (GO), and phosphoglycolate phosphatase (PGLP) under low O2 or with AOA confirmed the lowering of photorespiratory metabolism. A marked increase in ROS levels (superoxide and H2O2) indicated the induction of oxidative stress. Thus, our results demonstrated for the first time that restricted photorespiratory conditions increased the extent of oxidative stress. We propose that photorespiration is essential to sustain normal ROS levels and optimize metabolism in cellular compartments of Arabidopsis leaves. Supplementary Information The online version contains supplementary material available at 10.1007/s12298-023-01388-4.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Physiol Mol Biol Plants
Año:
2023
Tipo del documento:
Article
Pais de publicación:
India