Rational Biomimetic Construction of Lignin-based Carbon Nanozyme for Identification of Uric Acid in Human Urine.
Talanta
; 271: 125657, 2024 May 01.
Article
en En
| MEDLINE
| ID: mdl-38218056
ABSTRACT
Nanozymes have made remarkable progress in the field of sensing assays by replacing native enzyme functions. However, it is still a challenge to rationally design active centers from molecular structure to enhance the catalytic performance and develop low-cost nanozymes. In this work, guided by the catalytic site of horseradish peroxidase (HRP), iron source and histidine were coupled to the main chain of aminated sodium lignosulfonate (SL) through the self-assembly biomimetic strategy to construct His-SL-Fe with peroxidase activity. The inherent functional groups and basic framework of aminated SL provide a robust environment and promote the formation of active sites. His-SL-Fe shows excellent robustness over multiple test cycles and has a strong affinity for the substrate compared to HRP. His-SL-Fe had been effectively integrated in the sensing system for catalytic detection of uric acid (UA) to achieve accurate recognition of UA in the range of 0.5-100 µM with the limit of detection as low as 0.18 µM. The recovery of human urine samples is in the range of 96.8%-106.1 % and the error is within 4 %. This work not only provides a new approach for the directed design of high-performance nanozymes, but also demonstrates promising ideas for the refined application of biomass resources.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Úrico
/
Carbono
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Talanta
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos