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Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts: Friend or foe?
Arykbaeva, Asel S; Lerink, Lente J S; Vos, Jaël; Engelse, Marten A; van Kooten, Cees; de Korte, Dirk; Lagerberg, Johan W M; Klei, Thomas R L; Mulder, Aat A; Minnee, Robert C; Ploeg, Rutger J; Kers, Jesper; Moers, Cyril; Pol, Robert A; Alwayn, Ian P J; de Vries, Dorottya K; Lindeman, Jan H N.
Afiliación
  • Arykbaeva AS; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.
  • Lerink LJS; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.
  • Vos J; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.
  • Engelse MA; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands; Nuffield Department of Surgical Sciences, University of Oxford, Oxford, UK.
  • van Kooten C; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands; Nuffield Department of Surgical Sciences, University of Oxford, Oxford, UK.
  • de Korte D; Division of Nephrology and Transplant Medicine, Leiden University Medical Center, Leiden, The Netherlands.
  • Lagerberg JWM; Division of Nephrology and Transplant Medicine, Leiden University Medical Center, Leiden, The Netherlands.
  • Klei TRL; Division of Nephrology and Transplant Medicine, Leiden University Medical Center, Leiden, The Netherlands.
  • Mulder AA; Department of Product and Process Development, Sanquin Blood Bank, Amsterdam, The Netherlands.
  • Minnee RC; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands.
  • Ploeg RJ; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands; Department of Surgery, Division of Hepatopancreatobiliary and Transplant Surgery, Erasmus MC Transplant Institute, University Medical Center Rotterdam, Rotterdam, The Netherlands.
  • Kers J; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands; Department of Pathology, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands; Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands; Van 't Hoff Institute for Molecular Sciences,
  • Moers C; Department of Surgery - Organ Donation and Transplantation, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
  • Pol RA; Department of Surgery - Organ Donation and Transplantation, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
  • Alwayn IPJ; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.
  • de Vries DK; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.
  • Lindeman JHN; Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: j.h.n.lindeman@lumc.nl.
Am J Transplant ; 24(7): 1172-1179, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38215981
ABSTRACT
Renal ex vivo normothermic machine perfusion (NMP) is under development as an assessment tool for high-risk kidney grafts and as a means of achieving more physiologically accurate organ preservation. On-going hemolysis has been reported during NMP, as this technique relies on red blood cells for oxygen delivery. In this study, we confirm the occurrence of progressive hemolysis during 6-hour kidney NMP. NMP-associated erythrostasis in the glomeruli and in peri-glomerular vascular networks points to an interaction between the red blood cells and the graft. Continuous hemolysis resulted in prooxidative changes in the perfusate, which could be quenched by addition of fresh frozen plasma. In a cell-based system, this hemolysis induced redox stress and exhibited toxic effects at high concentrations. These findings highlight the need for a more refined oxygen carrier in the context of renal NMP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Órganos / Oxígeno / Perfusión / Trasplante de Riñón / Eritrocitos Límite: Animals / Humans / Male Idioma: En Revista: Am J Transplant Asunto de la revista: TRANSPLANTE Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Órganos / Oxígeno / Perfusión / Trasplante de Riñón / Eritrocitos Límite: Animals / Humans / Male Idioma: En Revista: Am J Transplant Asunto de la revista: TRANSPLANTE Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos