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A novel characterized multi-drug-resistant Pseudocitrobacter sp. isolated from a patient colonized while admitted to a tertiary teaching hospital.
Kelly, S A; O'Connell, N H; Thompson, T P; Dillon, L; Wu, J; Creevey, C; Powell, J; Gilmore, B F; Dunne, C P.
Afiliación
  • Kelly SA; School of Pharmacy, Queen's University Belfast, Belfast, UK.
  • O'Connell NH; Department of Clinical Microbiology, University Hospital Limerick, Limerick, Ireland; School of Medicine and Centre for Interventions in Infection, Inflammation, and Immunity, University of Limerick, Limerick, Ireland.
  • Thompson TP; School of Pharmacy, Queen's University Belfast, Belfast, UK.
  • Dillon L; School of Biological Sciences, Queen's University Belfast, Belfast, UK.
  • Wu J; School of Pharmacy, Queen's University Belfast, Belfast, UK.
  • Creevey C; School of Biological Sciences, Queen's University Belfast, Belfast, UK.
  • Powell J; Department of Clinical Microbiology, University Hospital Limerick, Limerick, Ireland; School of Medicine and Centre for Interventions in Infection, Inflammation, and Immunity, University of Limerick, Limerick, Ireland.
  • Gilmore BF; School of Pharmacy, Queen's University Belfast, Belfast, UK.
  • Dunne CP; School of Medicine and Centre for Interventions in Infection, Inflammation, and Immunity, University of Limerick, Limerick, Ireland. Electronic address: colum.dunne@ul.ie.
J Hosp Infect ; 145: 193-202, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38215945
ABSTRACT

BACKGROUND:

Reports of nosocomial infections typically describe recognised microorganisms. Here, a novel bacterial species was isolated, based on rectal swab screening for carbapenemases post-admission, then phenotypically and genetically characterized.

METHODS:

Sensititre, Vitek and API kits, MALDI and Illumina MiSeq were employed before profiles and phylogeny were compared with other related species.

FINDINGS:

Determined to be a possible Enterobacterales, the isolate was found to have 99.7% 16s rRNA identity to Pseudocitrobacter corydidari; an Asian cockroach-associated species. Given the highly conserved/low variability of 16S rRNA genes in Enterobacterales, average nucleotide identity (ANI) analysis compared the new isolate's genome with those of 18 Enterobacteriaceae species, including confirmed species of Pseudocitrobacter and unnamed Pseudocitrobacter species in the SILVA database. Of these, Pseudocitrobactercorydidari had the highest ANI at 0.9562. The published genome of the only known isolate of P.corydidari does not include Antimicrobial Resistance Genes (ARGs), with exception of potential drug efflux transporters. In contrast, our clinical isolate bears recognised antimicrobial resistance genes, including Klebsiella pneumoniae carbapenemase. The associated genome suggests resistance to carbapenems, ß-lactams, sulfonamides, fluoroquinolones, macrolides, aminoglycosides and cephalosporins. Phenotypic antimicrobial resistance was confirmed.

CONCLUSION:

Evident variations in ARG profiles, human colonization and origin in a clinically relevant niche that is geographically, physically and chemically disparate lend credibility for divergent evolution or, less likely, parallel evolution with P. corydidari. Genome data for this new species have been submitted to GENBANK using the proposed nomenclature Pseudocitrobacter limerickensis. The patient was colonized, rather than infected, and did not require antimicrobial treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enterobacteriaceae / Antibacterianos Límite: Humans Idioma: En Revista: J Hosp Infect Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enterobacteriaceae / Antibacterianos Límite: Humans Idioma: En Revista: J Hosp Infect Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido