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Modeling Foam Cell Formation in A Hydrogel-Based 3D-Intimal Model: A Study of The Role of Multi-Diseases During Early Atherosclerosis.
Akther, Fahima; Sajin, Dimple; Moonshi, Shehzahdi S; Wu, Yuao; Vazquez-Prada, Karla X; Ta, Hang Thu.
Afiliación
  • Akther F; Queensland Micro- and Nanotechnology, Griffith University, Nathan, Queensland, 4111, Australia.
  • Sajin D; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, Queensland, 4072, Australia.
  • Moonshi SS; Queensland Micro- and Nanotechnology, Griffith University, Nathan, Queensland, 4111, Australia.
  • Wu Y; Queensland Micro- and Nanotechnology, Griffith University, Nathan, Queensland, 4111, Australia.
  • Vazquez-Prada KX; Queensland Micro- and Nanotechnology, Griffith University, Nathan, Queensland, 4111, Australia.
  • Ta HT; Queensland Micro- and Nanotechnology, Griffith University, Nathan, Queensland, 4111, Australia.
Adv Biol (Weinh) ; 8(4): e2300463, 2024 04.
Article en En | MEDLINE | ID: mdl-38200677
ABSTRACT
Monocyte recruitment and transmigration are crucial in atherosclerotic plaque development. The multi-disease complexities aggravate the situation and continue to be a constant concern for understanding atherosclerosis plaque development. Herein, a 3D hydrogel-based model that integrates disease-induced microenvironments is sought to be designed, allowing us to explore the early stages of atherosclerosis, specifically examining monocyte fate in multi-disease complexities. As a proof-of-concept study, murine cells are employed to develop the model. The model is constructed with collagen embedded with murine aortic smooth muscle cells and a murine endothelial monolayer lining. The model achieves in vitro disease complexities using external stimuli such as glucose and lipopolysaccharide (LPS). Hyperglycemia exhibits a significant increase in monocyte adhesion but no enhancement in monocyte transmigration and foam cell conversion compared to euglycemia. Chronic infection achieved by LPS stimulation results in a remarkable augment in initial monocyte attachment and a significant increment in monocyte transmigration and foam cells in all concentrations. Moreover, the model exhibits synergistic sensitivity under multi-disease conditions such as hyperglycemia and infection, enhancing initial monocyte attachment, cell transmigration, and foam cell formation. Additionally, western blot data prove the enhanced levels of inflammatory biomarkers, indicating the model's capability to mimic disease-induced complexities during early atherosclerosis progression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aterosclerosis / Placa Aterosclerótica / Hiperglucemia Límite: Animals Idioma: En Revista: Adv Biol (Weinh) Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aterosclerosis / Placa Aterosclerótica / Hiperglucemia Límite: Animals Idioma: En Revista: Adv Biol (Weinh) Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Alemania