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In vitro co-expression chromatin assembly and remodeling platform for plant histone variants.
Banko, Petra; Okimune, Kei-Ichi; Nagy, Szilvia K; Hamasaki, Akinori; Morishita, Ryo; Onouchi, Hitoshi; Takasuka, Taichi E.
Afiliación
  • Banko P; Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
  • Okimune KI; Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
  • Nagy SK; Graduate School of Global Food Resources, Hokkaido University, Sapporo, 060-0809, Japan.
  • Hamasaki A; Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
  • Morishita R; Department of Molecular Biology, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, 1094, Hungary.
  • Onouchi H; CellFree Sciences Co., Ltd, Matsuyama, 790-8577, Japan.
  • Takasuka TE; CellFree Sciences Co., Ltd, Matsuyama, 790-8577, Japan.
Sci Rep ; 14(1): 936, 2024 01 10.
Article en En | MEDLINE | ID: mdl-38195981
ABSTRACT
Histone variants play a central role in shaping the chromatin landscape in plants, yet, how their distinct combinations affect nucleosome properties and dynamics is still largely elusive. To address this, we developed a novel chromatin assembly platform for Arabidopsis thaliana, using wheat germ cell-free protein expression. Four canonical histones and five reported histone variants were used to assemble twelve A. thaliana nucleosome combinations. Seven combinations were successfully reconstituted and confirmed by supercoiling and micrococcal nuclease (MNase) assays. The effect of the remodeling function of the CHR11-DDR4 complex on these seven combinations was evaluated based on the nucleosome repeat length and nucleosome spacing index obtained from the MNase ladders. Overall, the current study provides a novel method to elucidate the formation and function of a diverse range of nucleosomes in plants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nucleosomas / Arabidopsis Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nucleosomas / Arabidopsis Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido