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Near-Infrared Fluorescence Probe for Monoamine Oxidase A with a Large Stokes Shift for Intraoperative Navigation.
Zhang, Linhao; Zhang, Rong; Cong, Xinyue; He, Maomao; Zhao, Xin; Fan, Jiangli; Peng, Xiaojun; Cui, Jingnan; Sun, Wen.
Afiliación
  • Zhang L; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
  • Zhang R; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
  • Cong X; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
  • He M; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
  • Zhao X; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
  • Fan J; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
  • Peng X; Ningbo Institute of Dalian University of Technology, Ningbo 315016, China.
  • Cui J; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
  • Sun W; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
ACS Appl Bio Mater ; 7(2): 1115-1124, 2024 02 19.
Article en En | MEDLINE | ID: mdl-38194480
ABSTRACT
Monoamine oxidase A (MAO-A) is a dimeric flavoprotein that is found in the mitochondrial membrane. Currently, there is a lack of near-infrared fluorescent probes (NIR-FPs) with good specificity and high sensitivity for detecting MAO-A, making it difficult to accurately recognize and image cells in vitro and in vivo. In this study, the NIR-FP DDM-NH2 was designed and synthesized in order to detect MAO-A specifically in live biological systems. The probe comprised two functional components dicyanoisophosphone as an NIR dye precursor and alanine as a recognition moiety. After identifying MAO-A, the probe exhibited an NIR emission peak at 770 nm with a significant Stokes shift (180 nm), 11-fold response factor, low detection limit of 99.7 nM, and considerably higher affinity toward MAO-A than that toward MAO-B, indicating high sensitivity. In addition, DDM-NH2 was effective when applied to the image-based assessment of MAO-A activity in HeLa cells, zebrafish, and tumor-bearing mice, demonstrating great potential for visualization-based research and MAO-A application in vivo.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Monoaminooxidasa Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: ACS Appl Bio Mater Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Monoaminooxidasa Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: ACS Appl Bio Mater Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos