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Phylogenomics of the psychoactive mushroom genus Psilocybe and evolution of the psilocybin biosynthetic gene cluster.
Bradshaw, Alexander J; Ramírez-Cruz, Virginia; Awan, Ali R; Furci, Giuliana; Guzmán-Dávalos, Laura; Dentinger, Bryn T M.
Afiliación
  • Bradshaw AJ; School of Biological Sciences, University of Utah, Salt Lake City, UT 84112.
  • Ramírez-Cruz V; Natural History Museum of Utah, Collections and Research, University of Utah, Salt Lake City, UT 84108.
  • Awan AR; Consejo Nacional de Humanidades, Ciencias y Tecnologías (CONAHCYT), Departamento de Botánica y Zoología, Universidad de Guadalajara, Zapopan 45147, Mexico.
  • Furci G; Genomics Innovation Unit, Guy's and St.Thomas' NHS Foundation Trust, St Thomas' Hospital, London SE1 7EH, United Kingdom.
  • Guzmán-Dávalos L; Fungi Foundation, Brooklyn, NY 11216.
  • Dentinger BTM; Departamento de Botánica y Zoología, Universidad de Guadalajara, Zapopan 45147, Mexico.
Proc Natl Acad Sci U S A ; 121(3): e2311245121, 2024 Jan 16.
Article en En | MEDLINE | ID: mdl-38194448
ABSTRACT
Psychoactive mushrooms in the genus Psilocybe have immense cultural value and have been used for centuries in Mesoamerica. Despite the recent surge of interest in these mushrooms due to the psychotherapeutic potential of their natural alkaloid psilocybin, their phylogeny and taxonomy remain substantially incomplete. Moreover, the recent elucidation of the psilocybin biosynthetic gene cluster is known for only five of ~165 species of Psilocybe, four of which belong to only one of two major clades. We set out to improve the phylogeny of Psilocybe using shotgun sequencing of fungarium specimens, from which we obtained 71 metagenomes including from 23 types, and conducting phylogenomic analysis of 2,983 single-copy gene families to generate a fully supported phylogeny. Molecular clock analysis suggests the stem lineage of Psilocybe arose ~67 mya and diversified ~56 mya. We also show that psilocybin biosynthesis first arose in Psilocybe, with 4 to 5 possible horizontal transfers to other mushrooms between 40 and 9 mya. Moreover, predicted orthologs of the psilocybin biosynthetic genes revealed two distinct gene orders within the biosynthetic gene cluster that corresponds to a deep split within the genus, possibly a signature of two independent acquisitions of the cluster within Psilocybe.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agaricales / Psilocybe Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agaricales / Psilocybe Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos