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Salivary alpha-amylase stress reactivity in advanced-aged marmosets (Callithrix jacchus): Impacts of cognitive function and oral health status.
Sarmet, Max; Takehara, Sachiko; de Campos, Priscila S; Kagiyama, Kensuke; Borges, Luana S; Kumei, Yasuhiro; Zeredo, Jorge L L.
Afiliación
  • Sarmet M; Graduate Program in Health Sciences and Technologies, University of Brasilia, Brasilia, Brazil.
  • Takehara S; Division of Preventive Dentistry, Department of Oral Health Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan.
  • de Campos PS; Breathing Research and Therapeutics Center, University of Florida, Gainesville, Florida, USA.
  • Kagiyama K; CLEA Japan Inc., Gifu, Japan.
  • Borges LS; Graduate Program in Health Sciences and Technologies, University of Brasilia, Brasilia, Brazil.
  • Kumei Y; Department of Pathological Biochemistry, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
  • Zeredo JLL; Graduate Program in Health Sciences and Technologies, University of Brasilia, Brasilia, Brazil.
Am J Primatol ; 86(4): e23596, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38192065
ABSTRACT
Salivary alpha-amylase (sAA) is an enzyme found in saliva and is considered a noninvasive biomarker for sympathetic nervous system activity. While a wide range of sAA activity in response to stress has been reported in nonhuman primates, the effects of stress on sAA activity in common marmosets are still unknown. We tested the hypothesis that advanced age and cognitive function may have an impact on stress-related sAA reactivity in marmosets. Thirteen marmosets (nine males and five females) had saliva samples collected during a stressful condition (manual restraint stress) at two different time points, with a 60-min interval. On the next day, the animals underwent the object recognition test (ORT, a type of cognitive test), and then oral examinations. The animals were categorized into two age groups old (10-13 years), and very old (15-22 years). Irrespective of age, sAA levels showed a significant difference between T1 (mean 2.07 ± 0.86 U/mL) and T2 samples (mean 1.03 ± 0.67 U/mL), with higher values observed at T1 (p < 0.001). The intra-assay coefficients of variation (CV) for low and high sAA concentrations were 10.79% and 8.17%, respectively, while the interassay CVs for low and high sAA concentrations were 6.39% and 4.38%, respectively. Oral health issues were common but did not significantly impact sAA levels. The ORT indicated that the animals could recognize an object placed in the cage 6 h after familiarization. In conclusion, all marmosets showed a higher sAA concentration in the first saliva sample as compared to the second saliva sample collected 1 h later, indicating adaptation to stress. No significant differences in sAA levels were observed between sexes, ORT performance, or oral health. Our results indicate that autonomic responsivity and cognitive (memory) functions were preserved even in very old marmosets.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alfa-Amilasas Salivales Aspecto: Patient_preference Límite: Animals Idioma: En Revista: Am J Primatol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alfa-Amilasas Salivales Aspecto: Patient_preference Límite: Animals Idioma: En Revista: Am J Primatol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos