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Pre-fertilization approach using α-l-fucosidase modulates zona pellucida hardening during bovine in vitro embryo production.
Manssur, Thainá Sallum Bacco; Sebastião, Talita Raquel Cavichioli; Franchi, Fernanda Fagali; Dos Santos, Priscila Helena; Razza, Eduardo Montanari; Nunes, Sarah Gomes; Castilho, Anthony César de Souza; Fontes, Patricia Kubo.
Afiliación
  • Manssur TSB; University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo State, Brazil.
  • Sebastião TRC; University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo State, Brazil.
  • Franchi FF; Laboratory of Phytomedicines, Pharmacology and Biotechnology, Department of Pharmacology, Institute of Biosciences, University of São Paulo State (UNESP), Rua Prof. Antonio Celso Wagner Zanin, S/N, Zip Code: 18618689, Botucatu, São Paulo State, Brazil.
  • Dos Santos PH; Laboratory of Phytomedicines, Pharmacology and Biotechnology, Department of Pharmacology, Institute of Biosciences, University of São Paulo State (UNESP), Rua Prof. Antonio Celso Wagner Zanin, S/N, Zip Code: 18618689, Botucatu, São Paulo State, Brazil.
  • Razza EM; Laboratory of Phytomedicines, Pharmacology and Biotechnology, Department of Pharmacology, Institute of Biosciences, University of São Paulo State (UNESP), Rua Prof. Antonio Celso Wagner Zanin, S/N, Zip Code: 18618689, Botucatu, São Paulo State, Brazil.
  • Nunes SG; Laboratory of Phytomedicines, Pharmacology and Biotechnology, Department of Pharmacology, Institute of Biosciences, University of São Paulo State (UNESP), Rua Prof. Antonio Celso Wagner Zanin, S/N, Zip Code: 18618689, Botucatu, São Paulo State, Brazil.
  • Castilho ACS; University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo State, Brazil.
  • Fontes PK; Laboratory of Phytomedicines, Pharmacology and Biotechnology, Department of Pharmacology, Institute of Biosciences, University of São Paulo State (UNESP), Rua Prof. Antonio Celso Wagner Zanin, S/N, Zip Code: 18618689, Botucatu, São Paulo State, Brazil. pkfontes@gmail.com.
Vet Res Commun ; 48(2): 1135-1147, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38191818
ABSTRACT
The polyspermy occurrence is considerably lower under in vivo compared to in vitro embryo culture conditions, suggesting that the presence of some factors in the maternal environment is responsible for this. The α-L-fucosidase (FUCA) is a natural glycosidase present in the oviductal fluid, therefore, this study aimed at investigating the effect of adding FUCA to the hardening of the zona pellucida (ZP), polyspermy control, and embryonic yield and quality of bovine blastocysts produced in vitro. In the first experiment, the effect of FUCA (0.125 U/mL) was evaluated during the entire in vitro fertilization (IVF). However, it was demonstrated to be embryotoxic by completely inhibiting the blastocyst formation. In the second experiment, the FUCA (0.125 U/mL) was tested as short-term incubation before IVF (pre-fertilization step) for 30 min or 2 h, which demonstrated that FUCA treatment for 30 min resulted in ZP hardening. In the third experiment, a pre-fertilization FUCA treatment (1 h) at different concentrations (0, 0.0625, and 0.125 U/mL) showed that FUCA (0.0625 U/mL) improved pre-fertilization ZP hardening and tended to increase monospermic fertilization rates but did not improve embryo yield and quality. Together, it has been demonstrated that FUCA can induce oocyte pre-fertilization ZP hardening and might improve monospermic fertilization performance, and this effect is dependent on both variables (protein concentration and incubation time).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Zona Pelúcida / Alfa-L-Fucosidasa Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Zona Pelúcida / Alfa-L-Fucosidasa Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza