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Cultivation of Schwann cells from fresh and non-fresh adult equine peripheral nerves.
Kvigstad, Elise Friis; Øverland, Ingvild Ketilsdotter; Skedsmo, Fredrik Strebel; Jäderlund, Karin Hultin; Gröndahl, Gittan; Hanche-Olsen, Siv; Gunnes, Gjermund.
Afiliación
  • Kvigstad EF; Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, Ås 1433, Norway.
  • Øverland IK; Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, Ås 1433, Norway.
  • Skedsmo FS; Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, Ås 1433, Norway.
  • Jäderlund KH; Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oluf Thesensvei 24/30, Ås 1433, Norway.
  • Gröndahl G; Department of Animal Health and Microbial Strategies, National Veterinary Institute, Uppsala 75189, Sweden.
  • Hanche-Olsen S; Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oluf Thesensvei 24/30, Ås 1433, Norway.
  • Gunnes G; Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Elizabeth Stephansens vei 15, Ås 1433, Norway. Electronic address: gjermund.gunnes@nmbu.no.
J Neurosci Methods ; 403: 110054, 2024 03.
Article en En | MEDLINE | ID: mdl-38181868
ABSTRACT

BACKGROUND:

Over the past 25 years, acquired equine polyneuropathy (AEP) has emerged as a neurological disease in Scandinavian horses. This condition is characterized by histopathological features including the presence of Schwann cell (SC) inclusions. Cultivated equine SCs would serve as a valuable resource for investigations of factors triggering this Schwannopathy. Ideally, cells should be sampled for cultivation from fresh nerves immediately after death of the animal, however the availability of fresh material is limited, due to the inconsistent case load and the inherent technical and practical challenges to collection of samples in the field. This study aimed to cultivate SCs from adult equine peripheral nerves and assess their ability to survive in sampled nerve material over time to simulate harvesting of SCs in field situations. NEW

METHODS:

Peripheral nerves from five non-neurological horses were used. After euthanasia, both fresh and non-fresh nerve samples were harvested from each horse. Flow cytometry was employed to confirm the cellular identity and to determine the SC purity.

RESULTS:

The results revealed successful establishment of SC cultures from adult equine peripheral nerves, with the potential to achieve high SC purity from both fresh and non-fresh nerve samples. COMPARISON WITH EXISTING

METHOD:

While most SC isolation methods focus on harvest of cells from fresh nerve materials from laboratory animals, our approach highlights the possibility of utilizing SC cultures from field-harvested and transported nerve samples from horses.

CONCLUSIONS:

We describe a method for isolating SCs with high purity from both fresh and non-fresh peripheral nerves of adult horses.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervios Periféricos / Tejido Nervioso Límite: Animals Idioma: En Revista: J Neurosci Methods Año: 2024 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervios Periféricos / Tejido Nervioso Límite: Animals Idioma: En Revista: J Neurosci Methods Año: 2024 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Países Bajos