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Production, purification, and functional characterization of glucan exopolysaccharide produced by Enterococcus hirae strain OL616073 of fermented food origin.
Kavitake, Digambar; Tiwari, Swati; Devi, Palanisamy Bruntha; Shah, Irshad Ahmad; Reddy, G Bhanuprakash; Shetty, Prathapkumar Halady.
Afiliación
  • Kavitake D; Department of Biochemistry, National Institute of Nutrition, Hyderabad, Telangana 500007, India.
  • Tiwari S; Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India.
  • Devi PB; Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India.
  • Shah IA; Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India.
  • Reddy GB; Department of Biochemistry, National Institute of Nutrition, Hyderabad, Telangana 500007, India.
  • Shetty PH; Department of Food Science and Technology, Pondicherry University, Pondicherry 605014, India.. Electronic address: pkshalady@yahoo.co.uk.
Int J Biol Macromol ; 259(Pt 1): 129105, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38176508
ABSTRACT
Microbial exopolysaccharides (EPS) are high molecular weight polymeric substances with great diversity and variety of applications in the food and pharma industry. In this study, we report the extraction of an EPS from Enterococcus hirae OL616073 strain originally isolated from Indian fermented food and its purification by ion exchange and size exclusion chromatography for physical-functional analyses. The EPS showed two prominent fractions (EPS F1 and EPS F2) with molecular mass 7.7 × 104 and 6.5 × 104 Da respectively by gel permeation chromatography. These fractions were further characterized by FTIR, HPTLC, GC-MS, and NMR as a homopolysaccharide of glucose linked with α-(1 â†’ 6) and α-(1 â†’ 3) glycosidic linkages. The porous, spongy, granular morphology of EPS was observed under scanning electron microscopy. EPS has revealed strong physico-functional properties like water solubility index (76.75 %), water contact angle (65.74°), water activity (0.35), hygroscopicity (3.05 %), water holding capacity (296.19 %), oil holding capacity (379.91 %), foaming capacity (19.58 %), and emulsifying activity (EA1-72.22 %). Rheological analysis showed that aqueous solution of EPS exhibited a non-Newtonian fluid behavior and shear-thinning characteristics. Overall, EPS exhibits techno functional properties with potential applications as a functional biopolymer in food and pharma industry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enterococcus hirae / Glucanos Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enterococcus hirae / Glucanos Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos