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Organogenetic transcriptomes of the Drosophila embryo at single cell resolution.
Peng, Da; Jackson, Dorian; Palicha, Bianca; Kernfeld, Eric; Laughner, Nathaniel; Shoemaker, Ashleigh; Celniker, Susan E; Loganathan, Rajprasad; Cahan, Patrick; Andrew, Deborah J.
Afiliación
  • Peng D; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Jackson D; Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Palicha B; Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Kernfeld E; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Laughner N; Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Shoemaker A; Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Celniker SE; Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  • Loganathan R; Department of Biological Sciences, Wichita State University, Wichita, KS 67260, USA.
  • Cahan P; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
  • Andrew DJ; Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Development ; 151(2)2024 Jan 15.
Article en En | MEDLINE | ID: mdl-38174902
ABSTRACT
To gain insight into the transcription programs activated during the formation of Drosophila larval structures, we carried out single cell RNA sequencing during two periods of Drosophila embryogenesis stages 10-12, when most organs are first specified and initiate morphological and physiological specialization; and stages 13-16, when organs achieve their final mature architectures and begin to function. Our data confirm previous findings with regards to functional specialization of some organs - the salivary gland and trachea - and clarify the embryonic functions of another - the plasmatocytes. We also identify two early developmental trajectories in germ cells and uncover a potential role for proteolysis during germline stem cell specialization. We identify the likely cell type of origin for key components of the Drosophila matrisome and several commonly used Drosophila embryonic cell culture lines. Finally, we compare our findings with other recent related studies and with other modalities for identifying tissue-specific gene expression patterns. These data provide a useful community resource for identifying many new players in tissue-specific morphogenesis and functional specialization of developing organs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Development Asunto de la revista: BIOLOGIA / EMBRIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido