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Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration.
He, Ting; Wang, Yichun; Wang, Ruohan; Yang, Huan; Hu, Xueyi; Pu, Yiyao; Yang, Binbin; Zhang, Jingyuan; Li, Juan; Huang, Chongxiang; Jin, Rongrong; Nie, Yu; Zhang, Xingdong.
Afiliación
  • He T; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
  • Wang Y; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
  • Wang R; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
  • Yang H; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
  • Hu X; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
  • Pu Y; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
  • Yang B; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
  • Zhang J; Department of the Affiliated Stomatological Hospital of Southwest Medical University, Southwest Medical University, Luzhou 646000, China.
  • Li J; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
  • Huang C; State Key Laboratory of Oral Diseases, West China School of Stomatology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
  • Jin R; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
  • Nie Y; School of Aeronautics and Astronautics, Sichuan University, Chengdu 610064, China.
  • Zhang X; National Engineering Research Centre for Biomaterials/College of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
Regen Biomater ; 11: rbad111, 2024.
Article en En | MEDLINE | ID: mdl-38173764
ABSTRACT
Titanium (Ti) implants have been extensively used after surgical operations. Its surface bioactivity is of importance to facilitate integration with surrounding bone tissue, and ultimately ensure stability and long-term functionality of the implant. The plasmid DNA-activated matrix (DAM) coating on the surface could benefit osseointegration but is still trapped by poor transfection for further application, especially on the bone marrow mesenchymal stem cells (BMSCs) in vivo practical conditions. Herein, we constructed a DAM on the surface of fibrous-grained titanium (FG Ti) composed of phase-transition lysozyme (P) as adhesive, cationic arginine-rich lipid (RLS) as the transfection agent and plasmid DNA (pDNA) for bone morphology protein 2 (BMP2) expression. The cationic lipid RLS improved up to 30-fold higher transfection than that of commercial reagents (Lipofectamine 2000 and polyethyleneimine) on MSC. And importantly, Ti surface topology not only promotes the DAM to achieve high transfection efficiency (∼75.7% positive cells) on MSC due to the favorable combination but also reserves its contact induction effect for osteoblasts. Upon further exploration, the fibrous topology on FG Ti could boost pDNA uptake for gene transfection, and cell migration in MSC through cytoskeleton remodeling and induce contact guidance for enhanced osteointegration. At the same time, the cationic RLS together with adhesive P were both antibacterial, showing up to 90% inhibition rate against Escherichia coli and Staphylococcus aureus with reduced adherent microorganisms and disrupted bacteria. Finally, the FG Ti-P/pBMP2 implant achieved accelerated bone healing capacities through highly efficient gene delivery, aligned surface topological structure and increased antimicrobial properties in a rat femoral condylar defect model.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Regen Biomater Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Regen Biomater Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido