Your browser doesn't support javascript.
loading
Identifying distant relatives using benchtop-scale sequencing.
Woerner, August E; Novroski, Nicole M; Mandape, Sammed; King, Jonathan L; Crysup, Benjamin; Coble, Michael D.
Afiliación
  • Woerner AE; Center for Human Identification, University of North Texas Health Science Center, Fort Worth, TX, USA; Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX, USA. Electronic address: august.woerner@unthsc.edu.
  • Novroski NM; Center for Human Identification, University of North Texas Health Science Center, Fort Worth, TX, USA; Department of Anthropology, University of Toronto, Mississauga, ON, Canada.
  • Mandape S; Center for Human Identification, University of North Texas Health Science Center, Fort Worth, TX, USA.
  • King JL; Center for Human Identification, University of North Texas Health Science Center, Fort Worth, TX, USA.
  • Crysup B; Center for Human Identification, University of North Texas Health Science Center, Fort Worth, TX, USA; Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX, USA.
  • Coble MD; Center for Human Identification, University of North Texas Health Science Center, Fort Worth, TX, USA; Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX, USA.
Forensic Sci Int Genet ; 69: 103005, 2024 03.
Article en En | MEDLINE | ID: mdl-38171224
ABSTRACT
The genetic component of forensic genetic genealogy (FGG) is an estimate of kinship, often conducted at genome scales between a great number of individuals. The promise of FGG is substantial in concert with genealogical records and other nongenetic information, it can indirectly identify a person of interest. A downside of FGG is cost, as it is currently expensive and requires chemistries uncommon to forensic genetic laboratories (microarrays and high throughput sequencing). The more common benchtop sequencers can be coupled with a targeted PCR assay to conduct FGG, though such approaches have limited resolution for kinship. This study evaluates low-pass sequencing, an alternative strategy that is accessible to benchtop sequencers and can produce resolutions comparable to high-pass sequencing. Samples from a three-generation pedigree were augmented to include up to 7th degree relatives (using whole genome pedigree simulations) and the ability to recover the true kinship coefficient was assessed using algorithms qualitatively similar to those found in GEDmatch. We show that up to 7th degree relatives can be reliably inferred from 1 × whole genome sequencing obtainable from desktop sequencers.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algoritmos / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Humans Idioma: En Revista: Forensic Sci Int Genet Asunto de la revista: GENETICA / JURISPRUDENCIA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algoritmos / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Humans Idioma: En Revista: Forensic Sci Int Genet Asunto de la revista: GENETICA / JURISPRUDENCIA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos