Your browser doesn't support javascript.
loading
Cofilin-mediated actin filament network flexibility facilitates 2D to 3D actomyosin shape change.
Sun, Zachary Gao; Yadav, Vikrant; Amiri, Sorosh; Cao, Wenxiang; De La Cruz, Enrique M; Murrell, Michael.
Afiliación
  • Sun ZG; Systems Biology Institute, Yale University, West Haven, CT 06516, USA; Department of Physics, Yale University, 217 Prospect Street, New Haven, CT 06511, USA; Integrated Graduate Program in Physical and Engineering Biology, Yale University, New Haven, CT 06520, USA.
  • Yadav V; Systems Biology Institute, Yale University, West Haven, CT 06516, USA; Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA.
  • Amiri S; Systems Biology Institute, Yale University, West Haven, CT 06516, USA; Department of Mechanical Engineering and Material Science, Yale University, New Haven, CT 06511, USA.
  • Cao W; Department of Molecular Biology & Biophysics, Yale University, New Haven, CT 06511, USA.
  • De La Cruz EM; Department of Molecular Biology & Biophysics, Yale University, New Haven, CT 06511, USA.
  • Murrell M; Systems Biology Institute, Yale University, West Haven, CT 06516, USA; Department of Biomedical Engineering, Yale University, New Haven, CT 06511, USA; Department of Physics, Yale University, 217 Prospect Street, New Haven, CT 06511, USA; Integrated Graduate Program in Physical and Engineering Biolo
Eur J Cell Biol ; 103(1): 151379, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38168598
ABSTRACT
The organization of actin filaments (F-actin) into crosslinked networks determines the transmission of mechanical stresses within the cytoskeleton and subsequent changes in cell and tissue shape. Principally mediated by proteins such as α-actinin, F-actin crosslinking increases both network connectivity and rigidity, thereby facilitating stress transmission at low crosslinking yet attenuating transmission at high crosslinker concentration. Here, we engineer a two-dimensional model of the actomyosin cytoskeleton, in which myosin-induced mechanical stresses are controlled by light. We alter the extent of F-actin crosslinking by the introduction of oligomerized cofilin. At pH 6.5, F-actin severing by cofilin is weak, but cofilin bundles and crosslinks filaments. Given its effect of lowering the F-actin bending stiffness, cofilin- crosslinked networks are significantly more flexible and softer in bending than networks crosslinked by α-actinin. Thus, upon local activation of myosin-induced contractile stress, the network bends out-of-plane in contrast to the in-plane compression as observed with networks crosslinked by α-actinin. Here, we demonstrate that local effects on filament mechanics by cofilin introduces novel large-scale network material properties that enable the sculpting of complex shapes in the cell cytoskeleton.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Actinas / Factores Despolimerizantes de la Actina Tipo de estudio: Prognostic_studies Idioma: En Revista: Eur J Cell Biol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Actinas / Factores Despolimerizantes de la Actina Tipo de estudio: Prognostic_studies Idioma: En Revista: Eur J Cell Biol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania