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Phage Paride can kill dormant, antibiotic-tolerant cells of Pseudomonas aeruginosa by direct lytic replication.
Maffei, Enea; Woischnig, Anne-Kathrin; Burkolter, Marco R; Heyer, Yannik; Humolli, Dorentina; Thürkauf, Nicole; Bock, Thomas; Schmidt, Alexander; Manfredi, Pablo; Egli, Adrian; Khanna, Nina; Jenal, Urs; Harms, Alexander.
Afiliación
  • Maffei E; Biozentrum, University of Basel, Basel, Switzerland.
  • Woischnig AK; Institute of Food, Nutrition and Health, D-HEST, ETH Zurich, Zurich, Switzerland.
  • Burkolter MR; Laboratory of Infection Biology, Department of Biomedicine, University and University Hospital Basel, Basel, Switzerland.
  • Heyer Y; Biozentrum, University of Basel, Basel, Switzerland.
  • Humolli D; Institute of Food, Nutrition and Health, D-HEST, ETH Zurich, Zurich, Switzerland.
  • Thürkauf N; Biozentrum, University of Basel, Basel, Switzerland.
  • Bock T; Institute of Food, Nutrition and Health, D-HEST, ETH Zurich, Zurich, Switzerland.
  • Schmidt A; Biozentrum, University of Basel, Basel, Switzerland.
  • Manfredi P; Biozentrum, University of Basel, Basel, Switzerland.
  • Egli A; Biozentrum, University of Basel, Basel, Switzerland.
  • Khanna N; Biozentrum, University of Basel, Basel, Switzerland.
  • Jenal U; Division of Clinical Bacteriology and Mycology, University Hospital Basel, Basel, Switzerland.
  • Harms A; Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland.
Nat Commun ; 15(1): 175, 2024 Jan 02.
Article en En | MEDLINE | ID: mdl-38168031
ABSTRACT
Bacteriophages are ubiquitous viral predators that have primarily been studied using fast-growing laboratory cultures of their bacterial hosts. However, microbial life in nature is mostly in a slow- or non-growing, dormant state. Here, we show that diverse phages can infect deep-dormant bacteria and suspend their replication until the host resuscitates ("hibernation"). However, a newly isolated Pseudomonas aeruginosa phage, named Paride, can directly replicate and induce the lysis of deep-dormant hosts. While non-growing bacteria are notoriously tolerant to antibiotic drugs, the combination with Paride enables the carbapenem meropenem to eradicate deep-dormant cultures in vitro and to reduce a resilient bacterial infection of a tissue cage implant in mice. Our work might inspire new treatments for persistent bacterial infections and, more broadly, highlights two viral strategies to infect dormant bacteria (hibernation and direct replication) that will guide future studies on phage-host interactions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Pseudomonas / Bacteriófagos Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Pseudomonas / Bacteriófagos Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido