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3D printed porous membrane integrated devices to study the chemoattractant induced behavioural response of aquatic organisms.
Kalathil Balakrishnan, Hari; Schultz, Aaron G; Lee, Soo Min; Alexander, Richard; Dumée, Ludovic F; Doeven, Egan H; Yuan, Dan; Guijt, Rosanne M.
Afiliación
  • Kalathil Balakrishnan H; Centre for Rural and Regional Futures, Deakin University, Locked Bag 20000, Geelong, VIC 3320, Australia. Rosanne.Guijt@deakin.edu.au.
  • Schultz AG; Institute for Frontier Materials, Deakin University, Locked Bag 20000, Geelong, VIC 3320, Australia.
  • Lee SM; School of Life and Environmental Sciences, Deakin University, Locked Bag 20000, Geelong, VIC 3320, Australia.
  • Alexander R; Centre for Rural and Regional Futures, Deakin University, Locked Bag 20000, Geelong, VIC 3320, Australia. Rosanne.Guijt@deakin.edu.au.
  • Dumée LF; Centre for Rural and Regional Futures, Deakin University, Locked Bag 20000, Geelong, VIC 3320, Australia. Rosanne.Guijt@deakin.edu.au.
  • Doeven EH; Department of Chemical Engineering, Khalifa University, Abu Dhabi, United Arab Emirates.
  • Yuan D; Research and Innovation Centre on CO2 and Hydrogen, Khalifa University, Abu Dhabi, United Arab Emirates.
  • Guijt RM; School of Life and Environmental Sciences, Deakin University, Locked Bag 20000, Geelong, VIC 3320, Australia.
Lab Chip ; 24(3): 505-516, 2024 01 30.
Article en En | MEDLINE | ID: mdl-38165774
ABSTRACT
Biological models with genetic similarities to humans are used for exploratory research to develop behavioral screening tools and understand sensory-motor interactions. Their small, often mm-sized appearance raises challenges in the straightforward quantification of their subtle behavioral responses and calls for new, customisable research tools. 3D printing provides an attractive approach for the manufacture of custom designs at low cost; however, challenges remain in the integration of functional materials like porous membranes. Nanoporous membranes have been integrated with resin exchange using purpose-designed resins by digital light projection 3D printing to yield functionally integrated devices using a simple, economical and semi-automated process. Here, the impact of the layer thickness and layer number on the porous properties - parameters unique for 3D printing - are investigated, showing decreases in mean pore diameter and porosity with increasing layer height and layer number. From the same resin formulation, materials with average pore size between 200 and 600 nm and porosity between 45% and 61% were printed. Membrane-integrated devices were used to study the chemoattractant induced behavioural response of zebrafish embryos and planarians, both demonstrating a predominant behavioral response towards the chemoattractant, spending >85% of experiment time in the attractant side of the observation chamber. The presented 3D printing method can be used for printing custom designed membrane-integrated devices using affordable 3D printers and enable fine-tuning of porous properties through adjustment of layer height and number. This accessible approach is expected to be adopted for applications including behavioural studies, early-stage pre-clinical drug discovery and (environmental) toxicology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Organismos Acuáticos Límite: Animals / Humans Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Organismos Acuáticos Límite: Animals / Humans Idioma: En Revista: Lab Chip Asunto de la revista: BIOTECNOLOGIA / QUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido