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Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins.
Alves, Guilherme Guerra; Assis, Ronnie Antunes; do Amarante, Victor Santos; de Oliveira Júnior, Carlos Augusto; Silva, Rodrigo Otávio Silveira; Dias Heneine, Luiz Guilherme; Lobato, Francisco Carlos Faria.
Afiliación
  • Alves GG; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, MG, CEP 31.270-901, Brazil.
  • Assis RA; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, MG, CEP 31.270-901, Brazil.
  • do Amarante VS; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, MG, CEP 31.270-901, Brazil.
  • de Oliveira Júnior CA; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, MG, CEP 31.270-901, Brazil.
  • Silva ROS; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, MG, CEP 31.270-901, Brazil. Electronic address: rodrigo.otaviosilva@gmail.com.
  • Dias Heneine LG; Fundação Ezequiel Dias, Rua Conde Pereira Carneiro, 80 - Gameleira, Belo Horizonte, 30510-010, MG, Brazil.
  • Lobato FCF; Veterinary School, Universidade Federal de Minas Gerais (UFMG), Avenida Antônio Carlos, 6627, Belo Horizonte, MG, CEP 31.270-901, Brazil. Electronic address: lobato.francisco@yahoo.com.br.
Anaerobe ; 85: 102817, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38163631
ABSTRACT

OBJECTIVES:

This study aimed to produce and purify Clostridium perfringens type C beta-toxin, sheep anti-beta toxin immunoglobulin G (IgG) and chicken immunoglobulin Y (IgY).

METHODS:

Two methods were used for beta-toxin purification single-step metal affinity chromatography (MAC) using zinc as a chelator and ion exchange chromatography (IEX). The purified and inactivated beta-toxoids were then administered to sheep and chickens in order to produce IgG and IgY.

RESULTS:

All assays using the IEX failed. In contrast, MAC purified more than 21 mg of toxin per run in a single-step protocol. The purified and inactivated beta-toxoids were then administered to sheep and chickens, and IgG and IgY were purified with a high yield, medium antibody titer of 50 IU/mL, and high avidity (73.2 %).

CONCLUSIONS:

C. perfringens type C beta-toxin and sheep or chicken anti-beta toxin IgG and IgY antibodies were successfully produced and purified using a simple protocol. This protocol can be used for the production of components used in the diagnosis and research of necrotic enteritis caused by C. perfringens type C, as well as for the evaluation of existing vaccines and the development of new preventive methods against this disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Inmunoglobulinas / Antitoxinas / Infecciones por Clostridium / Enteritis Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Anaerobe Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Inmunoglobulinas / Antitoxinas / Infecciones por Clostridium / Enteritis Tipo de estudio: Guideline Límite: Animals Idioma: En Revista: Anaerobe Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido