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The pathogenicity of blood stream and central nervous system forms of Trypanosoma brucei rhodesiense trypanosomes in laboratory mice: a comparative study.
Ndungu, Kariuki; Thuita, John; Murilla, Grace; Kagira, John; Auma, Joanna; Mireji, Paul; Ngae, Geoffrey; Okumu, Paul; Gitonga, Purity; Guya, Samuel; Mdachi, Raymond.
Afiliación
  • Ndungu K; Biochemistry, Kenya Agricultural and Livestock Research Organization, Nairobi, P.O. Box 362 -00902, Kenya.
  • Thuita J; Animal Science, Meru University of Science and Technology, Meru, P.O Box, 972-60200, Kenya.
  • Murilla G; Administration, KAG East University, Nairobi, P.O.BOX 46328-00100, Kenya.
  • Kagira J; Animal Science, Jomo Keyatta University of Science and Technology, Nairobi, P.O. Box 62000-00200, Kenya.
  • Auma J; Biochemistry, Kenya Agricultural and Livestock Research Organization, Nairobi, P.O. Box 362 -00902, Kenya.
  • Mireji P; Bioinformatics, Centre for Geographic Medicine Research, Kilifi, P. O. Box 428-80108, Kenya.
  • Ngae G; Food Crops Research Institute, Kenya Agricultural and Livestock Research Organization, Nairobi, P. O. Box 30148-00200, Kenya.
  • Okumu P; Veterinary Pathology, University of Nairobi, Nairobi, P.O. Box 30197-00100, Kenya.
  • Gitonga P; Biochemistry, Kenya Agricultural and Livestock Research Organization, Nairobi, P.O. Box 362 -00902, Kenya.
  • Guya S; Biochemistry, Kenya Agricultural and Livestock Research Organization, Nairobi, P.O. Box 362 -00902, Kenya.
  • Mdachi R; Biochemistry, Kenya Agricultural and Livestock Research Organization, Nairobi, P.O. Box 362 -00902, Kenya.
F1000Res ; 11: 260, 2022.
Article en En | MEDLINE | ID: mdl-38162635
ABSTRACT

Background:

Human African trypanosomiasis (HAT) develops in two stages namely early stage when trypanosomes are found in the blood and late stage when trypanosomes are found in the central nervous system (CNS). The two environments are different with CNS environment reported as being hostile to the trypanosomes than the blood environment. The clinical symptoms manifested by the disease in the two environments are different. Information on whether blood stream are pathologically different from CNS trypanosomes is lacking. This study undertook to compare the inter-isolate pathological differences caused by bloodstream forms (BSF) and central nervous system (CNS) of five Trypanosoma brucei rhodesiense ( Tbr) isolates in Swiss white mice.

Methods:

Donor mice infected with each of the five isolates were euthanized at 21 days post infection (DPI) for recovery of BSF trypanosomes in heart blood and CNS trypanosomes in brain supernatants. Groups of Swiss white mice (n = 10) were then infected with BSF or CNS forms of each isolate and monitored for parasitaemia, packed cell volume (PCV), body weight, survivorship, trypanosome length, gross and histopathology characteristics.

Results:

Amplification of SRA gene prior to trypanosome morphology and pathogenicity studies confirmed all isolates as T. b. rhodesiense. At 21 DPI, CNS trypanosomes were predominantly long slender (LS) while BSF were a mixture of short stumpy and intermediate forms. The density of BSF trypanosomes was on average 2-3 log-scales greater than that of CNS trypanosomes with isolate KETRI 2656 having the highest CNS trypanosome density.

Conclusions:

The pathogenicity study revealed clear differences in the virulence/pathogenicity of the five (5) isolates but no distinct and consistent differences between CNS and BSF forms of the same isolate. We also identified KETRI 2656 as a suitable isolate for acute menigo- encephalitic studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma / Tripanosomiasis Africana Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: F1000Res Año: 2022 Tipo del documento: Article País de afiliación: Kenia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma / Tripanosomiasis Africana Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: F1000Res Año: 2022 Tipo del documento: Article País de afiliación: Kenia Pais de publicación: Reino Unido