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Pharmaceutical characterization and exploration of Arkeshwara rasa in MDA-MB-231 cells.
Jayakumar, Remya; Dash, Manoj Kumar; Kumar, Pankaj; Sharma, Shiwakshi; Gulati, Saumya; Pandey, Akanksha; Cholke, Kaushavi; Fatima, Zeeshan; Trigun, S K; Joshi, Namrata.
Afiliación
  • Jayakumar R; Department of Rasashastra and Bhaishajya Kalpana, Banaras Hindu University, Varanasi, 221005, India.
  • Dash MK; Department of Rasashastra and Bhaishajya Kalpana, Government Ayurveda College, Raipur, India. Electronic address: drmanojkumardash@gmail.com.
  • Kumar P; Department of Rasashastra and Bhaishajya Kalpana, Banaras Hindu University, Varanasi, 221005, India.
  • Sharma S; Department of Rasashastra and Bhaishajya Kalpana, Banaras Hindu University, Varanasi, 221005, India.
  • Gulati S; Dept of Rasashastra and Bhaishjya Kalpana, Babu Yugraj Singh Ayurvedic Medical College and Hospital, Gomtinagar Extension, Sector 6 Lucknow, Uttar Pradesh, 226010, India.
  • Pandey A; Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
  • Cholke K; Amity Lipidomics Research Facility (ALRF), Amity University, Haryana, Manesar, Gurugram, 122413, India; Institute of Biochemistry and Molecular Medicine, University of Bern, 3012, Switzerland.
  • Fatima Z; Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, 61922, Saudi Arabia; Amity Institute of Biotechnology, Amity University, Haryana, Manesar, Gurugram, 122413, India.
  • Trigun SK; Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
  • Joshi N; Department of Rasashastra and Bhaishajya Kalpana, Banaras Hindu University, Varanasi, 221005, India.
J Ayurveda Integr Med ; 15(1): 100823, 2024.
Article en En | MEDLINE | ID: mdl-38160612
ABSTRACT

BACKGROUND:

The diverse specificity mode of cancer treatment targets and chemo resistance demands the necessity of drug entities which can address the devastating dynamicity of the disease.

OBJECTIVES:

To check the anti-tumour potential of traditional medicine rich in polyherbal components and metal nanoparticle namely Arkeshwara rasa (AR). MATERIAL

METHODS:

The AR was prepared in a modified version with reference from Rasaratna Samuchaya and characterized using sophisticated instrumental analysis including XRD, SEM-EDAX, TEM, TGA-DSC, and LC-MS and tested against the MDA-MB-231 cell line to screen cell viability and the cytotoxicity with MTT, SRB and the AO assay.

RESULTS:

XRD pattern shows cubic tetrahedrite structure with Sb, Cu, S peaks and trace elements like Fe, Mg, etc. The particle size of AR ranges between 20 and 30 nm. The TGA points thermal decomposition at 210 °C and the metal sulphide peaks in DSC. LC-MS analysis reveals the components of the formulation more on the flavonoid portion. The IC50 value of MTT and SRB are 25.28 µg/mL and 31.7 µg/mL respectively. The AO colorimeter substantiated the cell viability and the apoptosis figures of the same cell line. The AR exhibits cytotoxicity and reaffirms the apoptosis fraction with SRB assay.

CONCLUSIONS:

The Hesperidine, Neohesperidin, Rutin components in the phytochemical pool can synergize the anti-tumour potential with either influencing cellular pathways or decreasing chemo resistance to conventional treatment. AR need to be further experimented with reverse transcription, flow cytometry, western blotting, etc.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Ayurveda Integr Med Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Ayurveda Integr Med Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos