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Electrochemistry detection of estrogenic effect: Regulation of de novo purine synthesis and catabolism by gibberellin and fulvestrant.
Wang, Yuhang; Zhao, Shuo; Wang, Shuhong; Zhang, Jing; Zhao, Yanli; Ye, Cai; Zhao, Zhiyu; Li, Jinlian; Shen, Hongkuan; Wu, Dongmei.
Afiliación
  • Wang Y; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, PR China.
  • Zhao S; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, PR China.
  • Wang S; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, PR China.
  • Zhang J; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, PR China.
  • Zhao Y; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, PR China; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, PR China.
  • Ye C; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, PR China; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, PR China.
  • Zhao Z; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, PR China.
  • Li J; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, PR China; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, PR China. Electronic address: lijinlian@jmsu.edu.cn.
  • Shen H; Jiamusi Inspection and Testing Center, Jiamusi, Heilongjiang 154007, PR China. Electronic address: 59387277@qq.com.
  • Wu D; College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, PR China; Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, Jiamusi University, Jiamusi 154007, PR China. Electronic address: dmwu@jmsu.edu.cn.
Bioelectrochemistry ; 156: 108634, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38160510
ABSTRACT
The estrogenic effect of plant growth regulators has been received little attention, which leads to the lack of relevant toxicity data. In this study, the estrogenic effect induced by gibberellin with ERα-dependent manner was found by E-screen and western blot methods, and the electrochemical signals of MCF-7 cells regulated by gibberellin and fulvestrant were investigated. The results showed that the electrochemical signals of MCF-7 cells were increased by gibberellin, while reduced by fulvestrant significantly, and displayed an extremely sensitive response to the effects of estrogenic effect induced by ERα agonist and antagonist. Western blot results showed that the expressions of phosphoribosyl pyrophosphate amidotransferase and hypoxanthine nucleotide dehydrogenase in de novo purine synthesis and adenine deaminase in catabolism were more effective regulated by gibberellin and fulvestrant, resulting in significant changes of the levels of guanine, hypoxanthine and xanthine in cells, and then electrochemical signals. The results provide a theoretical basis for the establishment of new electrochemical detection method of the estrogenic effect of plant regulators.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptor alfa de Estrógeno / Giberelinas Idioma: En Revista: Bioelectrochemistry Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptor alfa de Estrógeno / Giberelinas Idioma: En Revista: Bioelectrochemistry Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos