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Auxin-responsive ROS homeostasis genes display dynamic expression pattern during rice crown root primordia morphogenesis.
Kumar, Akshay; Verma, Komal; Kashyap, Rohan; Joshi, Vedika Jayant; Sircar, Debabrata; Yadav, Shri Ram.
Afiliación
  • Kumar A; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand, India.
  • Verma K; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand, India.
  • Kashyap R; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand, India.
  • Joshi VJ; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand, India.
  • Sircar D; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand, India.
  • Yadav SR; Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Uttarakhand, India. Electronic address: shri.yadav@bt.iitr.ac.in.
Plant Physiol Biochem ; 206: 108307, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38159549
ABSTRACT
Reactive oxygen species (ROS) are generated continuously as a by-product of aerobic metabolism in plants. While excessive ROS cause oxidative stresses in cells, they act as signaling molecules when maintained at an optimum concentration through the dynamic equilibrium of ROS metabolizing mechanisms to regulate growth, development and response to environmental stress. Auxin and its crosstalk with other signaling cascades are crucial for maintaining ROS homeostasis and orchestrating root architecture but dissecting the underlying mechanism requires detailed investigation at the molecular level. Rice fibrous root system is primarily composed of shoot-derived adventitious roots (also called crown roots). Here, we uncover auxin-ROS cross-talk during initiation and growth of rice roots. Potassium iodide treatment changes ROS levels that results in an altered rice root architecture. We reveal that auxin induction recover root growth and development defects by recouping level of hydrogen peroxide. By comparing global datasets previously generated by auxin induction and laser capture microdissection-RNA sequencing, we identify the redox-related antioxidants genes from peroxidase, glutathione reductase, glutathione S-transferase, and thioredoxin reductase families whose expression is regulated by the auxin signaling and also display dynamic expression patterns during crown root primordia morphogenesis. The auxin-mediated differential transcriptome data were validated by quantifying expression levels of a set of genes upon auxin induction. Further, in-depth spatio-temporal expression pattern analysis by RNA in situ hybridization shows the spatially restricted expression of selected genes in the developing crown root primordia. Together, our findings uncover molecular components of auxin-ROS crosstalk involved in root organogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Raíces de Plantas Límite: Humans Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Raíces de Plantas Límite: Humans Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Francia