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Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies.
Sandau, Ursula S; Magaña, Setty M; Costa, Júlia; Nolan, John P; Ikezu, Tsuneya; Vella, Laura J; Jackson, Hannah K; Moreira, Lissette Retana; Palacio, Paola Loreto; Hill, Andrew F; Quinn, Joseph F; Van Keuren-Jensen, Kendall R; McFarland, Trevor J; Palade, Joanna; Sribnick, Eric A; Su, Huaqi; Vekrellis, Kostas; Coyle, Beth; Yang, You; Falcón-Perez, Juan M; Nieuwland, Rienk; Saugstad, Julie A.
Afiliación
  • Sandau US; Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA.
  • Magaña SM; Center for Clinical and Translational Research, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, Ohio, USA.
  • Costa J; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Avenida da República, Oeiras, Portugal.
  • Nolan JP; Scintillon Institute for Biomedical and Bioenergy Research, San Diego, California, USA.
  • Ikezu T; Department of Neuroscience, Mayo Clinic Florida, Jacksonville, Florida, USA.
  • Vella LJ; Department of Surgery, The Royal Melbourne Hospital, The University of Melbourne, Parkville, Victoria, Australia.
  • Jackson HK; The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Melbourne, Victoria, Australia.
  • Moreira LR; Department of Pathology, University of Cambridge, Cambridge, UK.
  • Palacio PL; Exosis, Inc., Palm Beach, Florida, USA.
  • Hill AF; Department of Parasitology, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica, Central America.
  • Quinn JF; Centro de Investigación en Enfermedades Tropicales, University of Costa Rica, San José, Costa Rica, Central America.
  • Van Keuren-Jensen KR; Center for Clinical and Translational Research, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, Ohio, USA.
  • McFarland TJ; Institute for Health and Sport, Victoria University, Melbourne, Victoria, Australia.
  • Palade J; Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria, Australia.
  • Sribnick EA; Department of Neurology, Oregon Health & Science University, Portland, Oregon, USA.
  • Su H; Portland VA Medical Center, Portland, Oregon, USA.
  • Vekrellis K; Neurogenomics Division, Translational Genomics Research Institute, Phoenix, Arizona, USA.
  • Coyle B; Department of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA.
  • Yang Y; Neurogenomics Division, Translational Genomics Research Institute, Phoenix, Arizona, USA.
  • Falcón-Perez JM; Department of Neurosurgery, Nationwide Children's Hospital, The Ohio State University, Columbus, Ohio, USA.
  • Nieuwland R; The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, Melbourne, Victoria, Australia.
  • Saugstad JA; Biomedical Research Foundation Academy of Athens, Athens, Greece.
J Extracell Vesicles ; 13(1): e12397, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38158550
ABSTRACT
Cerebrospinal fluid (CSF) is a clear, transparent fluid derived from blood plasma that protects the brain and spinal cord against mechanical shock, provides buoyancy, clears metabolic waste and transports extracellular components to remote sites in the brain. Given its contact with the brain and the spinal cord, CSF is the most informative biofluid for studies of the central nervous system (CNS). In addition to other components, CSF contains extracellular vesicles (EVs) that carry bioactive cargoes (e.g., lipids, nucleic acids, proteins), and that can have biological functions within and beyond the CNS. Thus, CSF EVs likely serve as both mediators of and contributors to communication in the CNS. Accordingly, their potential as biomarkers for CNS diseases has stimulated much excitement for and attention to CSF EV research. However, studies on CSF EVs present unique challenges relative to EV studies in other biofluids, including the invasive nature of CSF collection, limited CSF volumes and the low numbers of EVs in CSF as compared to plasma. Here, the objectives of the International Society for Extracellular Vesicles CSF Task Force are to promote the reproducibility of CSF EV studies by providing current reporting and best practices, and recommendations and reporting guidelines, for CSF EV studies. To accomplish this, we created and distributed a world-wide survey to ISEV members to assess methods considered 'best practices' for CSF EVs, then performed a detailed literature review for CSF EV publications that was used to curate methods and resources. Based on responses to the survey and curated information from publications, the CSF Task Force herein provides recommendations and reporting guidelines to promote the reproducibility of CSF EV studies in seven domains (i) CSF Collection, Processing, and Storage; (ii) CSF EV Separation/Concentration; (iii) CSF EV Size and Number Measurements; (iv) CSF EV Protein Studies; (v) CSF EV RNA Studies; (vi) CSF EV Omics Studies and (vii) CSF EV Functional Studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Extracelulares Idioma: En Revista: J Extracell Vesicles Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vesículas Extracelulares Idioma: En Revista: J Extracell Vesicles Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos