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Identification of reproductive sex-biased gene expression in Asparagopsis taxiformis (lineage 6) gametophytes.
Patwary, Zubaida Parveen; Zhao, Min; Paul, Nicholas A; Cummins, Scott F.
Afiliación
  • Patwary ZP; Centre for Bioinnovation, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
  • Zhao M; School of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
  • Paul NA; Department of Aquaculture, Faculty of Fisheries, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
  • Cummins SF; School of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, Australia.
J Phycol ; 60(2): 327-342, 2024 04.
Article en En | MEDLINE | ID: mdl-38156746
ABSTRACT
The sub-tropical red seaweed Asparagopsis taxiformis is of significant interest due to its ability to store halogenated compounds, including bromoform, which can mitigate methane production in ruminants. Significant scale-up of aquaculture production of this seaweed is required; however, relatively little is known about the molecular mechanisms that control fundamental physiological processes, including the regulatory factors that determine sexual dimorphism in gametophytes. In this study, we used comparative RNA-sequencing analysis between different morphological parts of mature male and female A. taxiformis (lineage 6) gametophytes that resulted in greater number of sex-biased gene expression in tips (containing the reproductive structures for both sexes), compared with the somatic main axis and rhizomes. Further comparative RNA-seq against immature tips was used to identify 62 reproductive sex-biased genes (59 male-biased, 3 female-biased). Of the reproductive male-biased genes, 46% had an unknown function, while others were predicted to be regulatory factors and enzymes involved in signaling. We found that bromoform content obtained from female samples (8.5 ± 1.0 mg·g-1 dry weight) was ~10% higher on average than that of male samples (6.5 ± 1.0 mg·g-1 dry weight), although no significant difference was observed (p > 0.05). There was also no significant difference in the marine bromoform biosynthesis locus gene expression. In summary, our comparative RNA-sequencing analysis provides a first insight into the potential molecular factors relevant to gametogenesis and sexual differentiation in A. taxiformis, with potential benefits for identification of sex-specific markers.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algas Marinas / Rhodophyta / Células Germinativas de las Plantas Límite: Animals Idioma: En Revista: J Phycol Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Algas Marinas / Rhodophyta / Células Germinativas de las Plantas Límite: Animals Idioma: En Revista: J Phycol Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos