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[Investigation of oncolytic potential of vaccine strains of yellow fever and tick-borne encephalitis viruses against glioblastoma and pancreatic carcinoma cell lines].
Nazarenko, A S; Biryukova, Y K; Orlova, E O; Trachuk, K N; Ivanova, A L; Belyakova, A V; Pestov, N B; Vorovitch, M F; Ishmukhametov, A A; Kolyasnikova, N M.
Afiliación
  • Nazarenko AS; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis).
  • Biryukova YK; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis).
  • Orlova EO; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis).
  • Trachuk KN; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis).
  • Ivanova AL; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis).
  • Belyakova AV; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis).
  • Pestov NB; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis).
  • Vorovitch MF; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis).
  • Ishmukhametov AA; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University), Institute of Translational Medicine and Biotechnology.
  • Kolyasnikova NM; Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis).
Vopr Virusol ; 68(6): 536-548, 2023 Dec 26.
Article en Ru | MEDLINE | ID: mdl-38156569
ABSTRACT

INTRODUCTION:

Flaviviruses, possessing natural neurotropicity could be used in glioblastoma therapy using attenuated strains or as a delivery system for antitumor agents in an inactivated form.

OBJECTIVE:

To investigate the sensitivity of glioblastoma and pancreatic carcinoma cell lines to vaccine strains of yellow fever and tick-borne encephalitis viruses. MATERIALS AND

METHODS:

Cell lines glioblastoma GL-6, T98G, LN-229, pancreatic carcinoma MIA RaCa-2 and human pancreatic ductal carcinoma PANC-1. Viral strains 17D yellow fever virus (YF), Sofjin tick-borne encephalitis virus (TBEV). Virus concentration were determined by plaque assay and quantitative PCR. Determination of cell sensitivity to viruses by MTT assay.

RESULTS:

17D YF was effective only against pancreatic carcinoma tumor cells MIA Paca-2 and had a limited effect against PANC-1. In glioblastoma cell lines (LN229, GL6, T98G), virus had no oncolytic effect and the viral RNA concentration fell in the culture medium. Sofjin TBEV showed CPE50 against MIA Paca-2 and a very limited cytotoxic effect against PANC-1. However, it had no oncolytic effect against glioblastoma cell lines (LN229, T98G and GL6), although virus reproduction continued in these cultures. For the GL6 glioblastoma cell line, the viral RNA concentration at the level with the infection dose was determined within 13 days, despite medium replacement, while in the case of the LN229 cell line, the virus concentration increased from 1 × 109 to 1 × 1010 copies/ml.

CONCLUSION:

Tumor behavior in organism is more complex and is determined by different microenvironmental factors and immune status. In the future, it is advisable to continue studying the antitumor oncolytic and immunomodulatory effects of viral strains 17D YF and Sofjin TBEV using in vivo models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Fiebre Amarilla / Vacunas Virales / Glioblastoma / Encefalitis Transmitida por Garrapatas / Virus de la Encefalitis Transmitidos por Garrapatas Límite: Humans Idioma: Ru Revista: Vopr Virusol Año: 2023 Tipo del documento: Article Pais de publicación: Rusia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Fiebre Amarilla / Vacunas Virales / Glioblastoma / Encefalitis Transmitida por Garrapatas / Virus de la Encefalitis Transmitidos por Garrapatas Límite: Humans Idioma: Ru Revista: Vopr Virusol Año: 2023 Tipo del documento: Article Pais de publicación: Rusia