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Identification and Validation of Functional miRNAs and Their Main Targets in Sorghum bicolor.
Baqi, Abdul; Rehman, Wajid; Bibi, Iram; Menaa, Farid; Khan, Yousaf; Albalawi, Doha A; Sattar, Abdul.
Afiliación
  • Baqi A; Department of Chemistry, University of Balochistan, Quetta, 87300, Pakistan.
  • Samiullah; Department of Chemistry, University of Balochistan, Quetta, 87300, Pakistan.
  • Rehman W; Department of Chemistry, Hazara University Mansehra, Mansehra, Pakistan. sono_waj@yahoo.com.
  • Bibi I; Department of Chemistry, Hazara University Mansehra, Mansehra, Pakistan.
  • Menaa F; Department of Biomedical and Environmental Engineering (BEE), California Innovations Corporation (CIC), San Diego, CA, 92037, USA. menaateam@gmail.com.
  • Khan Y; Department of Chemistry, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
  • Albalawi DA; Department of Biology, Faculty of Science, University of Tabuk, 71491, Tabuk, Saudi Arabia.
  • Sattar A; Department of Chemistry, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
Mol Biotechnol ; 2023 Dec 28.
Article en En | MEDLINE | ID: mdl-38155285
ABSTRACT
MicroRNAs (miRNAs) are typically non-coding RNAs of 18-26 nucleotides (nts) that are produced endogenously and regulated post-transcriptionally through degradation or translational repression. Since miRNAs are evolutionarily conserved, their preservation is essential for important regulatory functions in plant development, growth, and responses to environmental stress. Sorghum bicolor (sbi) is a valuable food and fodder crop which is grown worldwide. A range of sbi miRNAs were identified so far as being connected to plant development and stress responses. Herein, we employed a variety of bioinformatics tools for miRNA profiling in sbi and a PCR-based platform for the validation of these miRNAs. In total, 74 new conserved sbi miRNAs from 52 miRNA families have been predicted. Using the psRNA Target method, 10613 different protein targets of these predicted miRNAs have been attained. These targets include 54 GO-terms which have substantial targets in the biological, molecular, and cellular processes. We particularly found that the sbi-miR1861c and sbi-miR5050 are involved to regulate sulphur compound biosynthetic process, while the significant spliceosomal complex is regulated by sbi-miR815b and sbi-miR7768b. Also, we report that the pre-ribosome, electron transport chain, cell communication, cellular respiration, protein localization, and photosynthesis are controlled by sbi-miR2907b, sbi-miR530, sbi-miR7749, sbi-miR1858a, sbi-mi7729a, and sbi-miR417, respectively. The identification and validation of these novel sbi miRNAs shall contribute a lot in improving the crop yield and ensure sustainable agriculture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mol Biotechnol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mol Biotechnol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Suiza