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Interactome Analysis Identifies the Role of BZW2 in Promoting Endoplasmic Reticulum-Mitochondria Contact and Mitochondrial Metabolism.
Maio, George; Smith, Mike; Bhawal, Ruchika; Zhang, Sheng; Baskin, Jeremy M; Li, Jenny; Lin, Hening.
Afiliación
  • Maio G; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, USA.
  • Smith M; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, USA.
  • Bhawal R; Proteomics and Metabolomics Facility, Cornell University, Ithaca, New York, USA.
  • Zhang S; Proteomics and Metabolomics Facility, Cornell University, Ithaca, New York, USA.
  • Baskin JM; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, USA; Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, New York, USA.
  • Li J; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, USA.
  • Lin H; Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York, USA; Howard Hughes Medical Institute, Cornell University, Ithaca, New York, USA; Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA. Electronic address: hl379@cornell.edu.
Mol Cell Proteomics ; 23(2): 100709, 2024 Feb.
Article en En | MEDLINE | ID: mdl-38154691
ABSTRACT
Understanding the molecular functions of less-studied proteins is an important task of life science research. Despite reports of basic leucine zipper and W2 domain-containing protein 2 (BZW2) promoting cancer progression first emerging in 2017, little is known about its molecular function. Using a quantitative proteomic approach to identify its interacting proteins, we found that BZW2 interacts with both endoplasmic reticulum (ER) and mitochondrial proteins. We thus hypothesized that BZW2 localizes to and promotes the formation of ER-mitochondria contact sites and that such localization would promote calcium transport from ER to the mitochondria and promote ATP production. Indeed, we found that BZW2 localized to ER-mitochondria contact sites and that BZW2 knockdown decreased ER-mitochondria contact, mitochondrial calcium levels, and ATP production. These findings provide key insights into molecular functions of BZW2, the potential role of BZW2 in cancer progression, and highlight the utility of interactome data in understanding the function of less-studied proteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Neoplasias Límite: Humans Idioma: En Revista: Mol Cell Proteomics Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Neoplasias Límite: Humans Idioma: En Revista: Mol Cell Proteomics Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos