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A Novel Adductomics Workflow Incorporating FeatureHunter Software: Rapid Detection of Nucleic Acid Modifications for Studying the Exposome.
Hu, Chiung-Wen; Chang, Yuan-Jhe; Chang, Wei-Hung; Cooke, Marcus S; Chen, Yet-Ran; Chao, Mu-Rong.
Afiliación
  • Hu CW; Department of Public Health, Chung Shan Medical University, Taichung 402, Taiwan.
  • Chang YJ; Department of Occupational Safety and Health, Chung Shan Medical University, Taichung 402, Taiwan.
  • Chang WH; Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan.
  • Cooke MS; Oxidative Stress Group, Department of Molecular Biosciences, University of South Florida, Tampa, Florida 33620, United States.
  • Chen YR; Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan.
  • Chao MR; Department of Occupational Safety and Health, Chung Shan Medical University, Taichung 402, Taiwan.
Environ Sci Technol ; 58(1): 75-89, 2024 Jan 09.
Article en En | MEDLINE | ID: mdl-38153287
ABSTRACT
Exposure to the physicochemical agents that interact with nucleic acids (NA) may lead to modification of DNA and RNA (i.e., NA modifications), which have been associated with various diseases, including cancer. The emerging field of NA adductomics aims to identify both known and unknown NA modifications, some of which may also be associated with proteins. One of the main challenges for adductomics is the processing of massive and complex data generated by high-resolution tandem mass spectrometry (HR-MS/MS). To address this, we have developed a software called "FeatureHunter", which provides the automated extraction, annotation, and classification of different types of key NA modifications based on the MS and MS/MS spectra acquired by HR-MS/MS, using a user-defined feature list. The capability and effectiveness of FeatureHunter was demonstrated by analyzing various NA modifications induced by formaldehyde or chlorambucil in mixtures of calf thymus DNA, yeast RNA and proteins, and by analyzing the NA modifications present in the pooled urines of smokers and nonsmokers. The incorporation of FeatureHunter into the NA adductomics workflow offers a powerful tool for the identification and classification of various types of NA modifications induced by reactive chemicals in complex biological samples, providing a valuable resource for studying the exposome.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / Exposoma Idioma: En Revista: Environ Sci Technol Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / Exposoma Idioma: En Revista: Environ Sci Technol Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos