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Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer.
Özyurt, Rumeysa; Erkasap, Nilüfer; Özkurt, Mete; Erkasap, Serdar; Dimas, Konstantinos; Çakir Gündogdu, Ayse; Ulukaya, Engin.
Afiliación
  • Özyurt R; Department of Experimental Therapeutics, MD Anderson Cancer Center, Houston, TX, USA.
  • Erkasap N; Department of Physiology, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkiye.
  • Özkurt M; Department of Physiology, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkiye.
  • Erkasap S; Department of Physiology, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkiye.
  • Dimas K; Department of General Surgery, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkiye.
  • Çakir Gündogdu A; Department of Pharmacology, School of Health Science, Thessaly University, Larissa, Greece.
  • Ulukaya E; Department of Histology and Embrology, Kütahya Health Sciences University Medical Faculty, Kütahya, Turkiye.
Turk J Biol ; 47(4): 290-300, 2023.
Article en En | MEDLINE | ID: mdl-38152619
ABSTRACT
Background/

aim:

Colorectal cancer (CRC) is a fatal malignancy type and its occurence still needs to be explored mechanistically. Notch, IL-1, and leptin crosstalk is reported to play a role in the proliferation, migration, and expression of proangiogenic molecules. In this study, we aimed to investigate the effect of inhibition of Notch, IL-1, and leptin on CRC. Materials and

methods:

To generate colorectal cancer tumor xenografts, 1 × 107 cells from exponentially growing cultures of HCT-15 cells were injected subcutaneously, at the axillary region of the left and right rear flanks of forty NOD.CB17-Prkdcscid/J (NOD/SCID) female mice. The mice were then monitored for the development of tumors and were randomly divided into five groups when tumor sizes reached a volume of approximately 150 mm3. Mice were used to determine the effectiveness of the gamma-secretase inhibitor (DAPT, Notch inhibitor), the interleukin-1 receptor antagonist (Anakinra) and the leptin receptor antagonist (Allo aca) against tumor growth. The mice were euthanized by CO2 inhalation immediately after the treatments finished, and all efforts were made to minimize suffering. Tumors were excissed for RT-PCR and histological analysis.

Results:

There is an intact Notch, IL-1, and leptin signaling axis, and in vivo antagonism of Notch, IL-1, and leptin affects mRNA and protein expression of inflammatory and angiogenic molecules.

Conclusion:

Present data suggest that targeting Notch, IL-1, and leptin may be possesses therapeutic potential in CRC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Turk J Biol Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Turquía
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Turk J Biol Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Turquía