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An iron fist in a velvet glove: The cooperation of a novel pyoverdine from Pseudomonas donghuensis P482 with 7-hydroxytropolone is pivotal for its antibacterial activity.
Jafra, Sylwia; Jablonska, Magdalena; Maciag, Tomasz; Matuszewska, Marta; Borowicz, Marcin; Prusinski, Michal; Zmudzinska, Wioletta; Thiel, Marcel; Czaplewska, Paulina; Krzyzanowska, Dorota M; Czajkowski, Robert.
Afiliación
  • Jafra S; Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Jablonska M; Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Maciag T; Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Matuszewska M; Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Borowicz M; Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Prusinski M; Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Zmudzinska W; Laboratory of Biopolymers Structure, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Thiel M; Laboratory of Biopolymers Structure, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Czaplewska P; Laboratory of Mass Spectrometry, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Krzyzanowska DM; Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
  • Czajkowski R; Laboratory of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology of the University of Gdansk and the Medical University of Gdansk, University of Gdansk, Gdansk, Poland.
Environ Microbiol ; 26(1): e16559, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38151794
ABSTRACT
Pseudomonas donghuensis P482 exhibits broad antimicrobial activity against phytopathogens, including the soft rot bacteria of the Dickeya genus. Here, we report that under limited nutrient availability, the antibacterial activity of P. donghuensis P482 against Dickeya solani requires the reciprocal action of two iron scavengers 7-hydroxytropolone (7-HT) and a newly characterized pyoverdine (PVDP482 ) and is quenched in the iron-augmented environment. Further, we show that the biosynthesis of pyoverdine and 7-HT is metabolically coordinated, and the functional BV82_4709 gene involved in 7-HT synthesis is pivotal for expressing the BV82_3755 gene, essential for pyoverdine biosynthesis and vice versa. The synthesis of both scavengers is under the control of Gac/Rsm, but only PVD is controlled by Fur. The isoelectric focusing profile of the P482 siderophore differs from that of the other Pseudomonas spp. tested. This finding led to the unveiling of the chemical structure of the new pyoverdine PVDP482 . To summarize, the antibacterial activity of P. donghuensis P482 is attributed to 7-HT and PVDP482 varies depending on the nutrient and iron availability, highlighting the importance of these factors in the competition between P482 and D. solani.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Pseudomonas / Tropolona / Hierro Idioma: En Revista: Environ Microbiol Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Pseudomonas / Tropolona / Hierro Idioma: En Revista: Environ Microbiol Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido