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Actuation of Soft Thermoresponsive Hydrogels Mechanically Stimulates Osteogenesis in Human Mesenchymal Stem Cells without Biochemical Factors.
Castro Nava, Arturo; Doolaar, Iris C; Labude-Weber, Norina; Malyaran, Hanna; Babu, Susan; Chandorkar, Yashoda; Di Russo, Jacopo; Neuss, Sabine; De Laporte, Laura.
Afiliación
  • Castro Nava A; DWI─Leibniz Institute for Interactive Materials, Forckenbeckstrasse 50, Aachen D-52074, Germany.
  • Doolaar IC; Institute for Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 1-2, Aachen D-52074, Germany.
  • Labude-Weber N; DWI─Leibniz Institute for Interactive Materials, Forckenbeckstrasse 50, Aachen D-52074, Germany.
  • Malyaran H; Institute for Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 1-2, Aachen D-52074, Germany.
  • Babu S; Helmholtz Institute for Biomedical Engineering, BioInterface Group, RWTH Aachen University, Pauwelsstrasse 20, Aachen D-52074, Germany.
  • Chandorkar Y; Helmholtz Institute for Biomedical Engineering, BioInterface Group, RWTH Aachen University, Pauwelsstrasse 20, Aachen D-52074, Germany.
  • Di Russo J; Interdisciplinary Centre for Clinical Research, RWTH Aachen University, Pauwelsstrasse 30, Aachen D-52074, Germany.
  • Neuss S; DWI─Leibniz Institute for Interactive Materials, Forckenbeckstrasse 50, Aachen D-52074, Germany.
  • De Laporte L; Institute for Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 1-2, Aachen D-52074, Germany.
ACS Appl Mater Interfaces ; 16(1): 30-43, 2024 Jan 10.
Article en En | MEDLINE | ID: mdl-38150508
ABSTRACT
Mesenchymal stem cells (MSCs) have the potential to differentiate into multiple lineages and can be harvested relatively easily from adults, making them a promising cell source for regenerative therapies. While it is well-known how to consistently differentiate MSCs into adipose, chondrogenic, and osteogenic lineages by treatment with biochemical factors, the number of studies exploring how to achieve this with mechanical signals is limited. A relatively unexplored area is the effect of cyclic forces on the MSC differentiation. Recently, our group developed a thermoresponsive N-ethyl acrylamide/N-isopropylacrylamide (NIPAM/NEAM) hydrogel supplemented with gold nanorods that are able to convert near-infrared light into heat. Using light pulses allows for local hydrogel collapse and swelling with physiologically relevant force and frequency. In this study, MSCs are cultured on this hydrogel system with a patterned surface and exposed to intermittent or continuous actuation of the hydrogel for 3 days to study the effect of actuation on MSC differentiation. First, cells are harvested from the bone marrow of three donors and tested for their MSC phenotype, meeting the following criteria the harvested cells are adherent and demonstrate a fibroblast-like bipolar morphology. They lack the expression of CD34 and CD45 but do express CD73, CD90, and CD105. Additionally, their differentiation potential into adipogenic, chondrogenic, and osteogenic lineages is validated by the addition of standardized differentiation media. Next, MSCs are exposed to intermittent or continuous actuation, which leads to a significantly enhanced cell spreading compared to nonactuated cells. Moreover, actuation results in nuclear translocation of Runt-related transcription factor 2 and the Yes-associated protein. Together, these results indicate that cyclic mechanical stimulation on a soft, ridged substrate modulates the MSC fate commitment in the direction of osteogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Células Madre Mesenquimatosas Límite: Adult / Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Células Madre Mesenquimatosas Límite: Adult / Humans Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos