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PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry.
Ouchida, Tsunenori; Tanaka, Tomohiro; Suzuki, Hiroyuki; Uchida, Kazuyuki; Nakagawa, Takayuki; Li, Guanjie; Nakamura, Takuro; Yanaka, Miyuki; Handa, Saori; Kaneko, Mika K; Kato, Yukinari.
Afiliación
  • Ouchida T; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Tanaka T; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Suzuki H; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Uchida K; Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
  • Nakagawa T; Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
  • Li G; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Nakamura T; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Yanaka M; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Handa S; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Kaneko MK; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
  • Kato Y; Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
Monoclon Antib Immunodiagn Immunother ; 42(6): 209-215, 2023 Dec.
Article en En | MEDLINE | ID: mdl-38150189
ABSTRACT
Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG1, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Jirafas / Anticuerpos Monoclonales Límite: Animals Idioma: En Revista: Monoclon Antib Immunodiagn Immunother Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Jirafas / Anticuerpos Monoclonales Límite: Animals Idioma: En Revista: Monoclon Antib Immunodiagn Immunother Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos