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Increased expression of chondroitin sulfate proteoglycans in dentate gyrus and amygdala causes postinfectious seizures.
Patel, Dipan C; Swift, Nathaniel; Tewari, Bhanu P; Browning, Jack L; Prim, Courtney; Chaunsali, Lata; Kimbrough, Ian F; Olsen, Michelle L; Sontheimer, Harald.
Afiliación
  • Patel DC; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA.
  • Swift N; School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
  • Tewari BP; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA.
  • Browning JL; School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
  • Prim C; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA.
  • Chaunsali L; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA.
  • Kimbrough IF; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA.
  • Olsen ML; School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
  • Sontheimer H; Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA.
Brain ; 147(5): 1856-1870, 2024 May 03.
Article en En | MEDLINE | ID: mdl-38146224
ABSTRACT
Alterations in the extracellular matrix are common in patients with epilepsy and animal models of epilepsy, yet whether they are the cause or consequence of seizures and epilepsy development is unknown. Using Theiler's murine encephalomyelitis virus (TMEV) infection-induced model of acquired epilepsy, we found de novo expression of chondroitin sulfate proteoglycans (CSPGs), a major extracellular matrix component, in dentate gyrus (DG) and amygdala exclusively in mice with acute seizures. Preventing the synthesis of CSPGs specifically in DG and amygdala by deletion of the major CSPG aggrecan reduced seizure burden. Patch-clamp recordings from dentate granule cells revealed enhanced intrinsic and synaptic excitability in seizing mice that was significantly ameliorated by aggrecan deletion. In situ experiments suggested that dentate granule cell hyperexcitability results from negatively charged CSPGs increasing stationary cations on the membrane, thereby depolarizing neurons, increasing their intrinsic and synaptic excitability. These results show increased expression of CSPGs in the DG and amygdala as one of the causal factors for TMEV-induced acute seizures. We also show identical changes in CSPGs in pilocarpine-induced epilepsy, suggesting that enhanced CSPGs in the DG and amygdala may be a common ictogenic factor and potential therapeutic target.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteoglicanos Tipo Condroitín Sulfato / Convulsiones / Giro Dentado / Amígdala del Cerebelo Límite: Animals Idioma: En Revista: Brain Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteoglicanos Tipo Condroitín Sulfato / Convulsiones / Giro Dentado / Amígdala del Cerebelo Límite: Animals Idioma: En Revista: Brain Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido