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Comprehensive analysis of biological landscape of oxidative stress-related genes in diabetic erectile dysfunction.
Meng, Qingjun; Chen, Yinwei; Cui, Lingang; Wei, Yinsheng; Li, Teng; Yuan, Penghui.
Afiliación
  • Meng Q; Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Chen Y; Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
  • Cui L; Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Wei Y; Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Li T; Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Yuan P; Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. yuanph2018@126.com.
Int J Impot Res ; 36(6): 627-635, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38145980
ABSTRACT
Oxidative stress plays a pivotal role in the pathogenesis of diabetic erectile dysfunction, while specific mechanisms have not been illuminated. The study aims to reveal the genetic expression patterns of oxidative stress in diabetic erectile dysfunction. Transcriptome data of diabetic erectile dysfunction and oxidative stress-related genes (OSRGs) in the Gene Expression Omnibus database were downloaded and analyzed based on differential expression. Functional enrichment analyses were conducted to clarify the biological functions. A protein interaction framework was established, and significant gene profiles were validated in the cavernous endothelial cells, clinical patients, and rat models. A miRNA-OSRGs network was predicted and validated. The results were analyzed using Student's t-test. The analysis screened 203 differentially expressed OSRGs (p < 0.05), which had a close association with oxidoreductase activities, glutathione metabolism, and autophagy. A four-gene signature comprised of EPS8L2 (p = 0.044), GSTA3 (p = 0.015), LOX (p < 0.001) and MGST1 (p = 0.002) was well validated and regarded as the hub OSRGs. Compared with the control group, notable increases and decreases were observed in the expressions of GSTA3 (3.683 ± 0.636 vs. 0.416 ± 0.507) and LOX (2.104 ± 1.895 vs. 18.804 ± 2.751) in the validated diabetic erectile dysfunction group. The hub OSRGs-related miRNAs participated in smooth muscle cell proliferation. Besides, miR-125a-3p (p = 0.034) and miR-138-2-3p (p = 0.012) were validated as promising oxidative stress-related miRNA biomarkers. Our findings revealed the genetic alternations of oxidative stress in diabetic erectile dysfunction. These results will be instructive to explore the molecular landscape and the potential treatment for diabetic erectile dysfunction.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Oxidativo / Disfunción Eréctil Límite: Animals / Humans / Male Idioma: En Revista: Int J Impot Res Asunto de la revista: MEDICINA REPRODUTIVA / UROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Oxidativo / Disfunción Eréctil Límite: Animals / Humans / Male Idioma: En Revista: Int J Impot Res Asunto de la revista: MEDICINA REPRODUTIVA / UROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido