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Direct assessment of nitrative stress in lipid environments: Applications of a designer lipid-based biosensor for peroxynitrite.
Gutierrez, Bryan; Aggarwal, Tushar; Erguven, Huseyin; Stone, M Rhia L; Guo, Changjiang; Bellomo, Alyssa; Abramova, Elena; Stevenson, Emily R; Laskin, Debra L; Gow, Andrew J; Izgu, Enver Cagri.
Afiliación
  • Gutierrez B; Department of Chemistry and Chemical Biology, Rutgers University, New Brunswick, NJ 08854, USA.
  • Aggarwal T; Department of Chemistry and Chemical Biology, Rutgers University, New Brunswick, NJ 08854, USA.
  • Erguven H; Department of Chemistry and Chemical Biology, Rutgers University, New Brunswick, NJ 08854, USA.
  • Stone MRL; Department of Chemistry and Chemical Biology, Rutgers University, New Brunswick, NJ 08854, USA.
  • Guo C; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA.
  • Bellomo A; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA.
  • Abramova E; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA.
  • Stevenson ER; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA.
  • Laskin DL; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA.
  • Gow AJ; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA.
  • Izgu EC; Department of Chemistry and Chemical Biology, Rutgers University, New Brunswick, NJ 08854, USA.
iScience ; 26(12): 108567, 2023 Dec 15.
Article en En | MEDLINE | ID: mdl-38144454
ABSTRACT
Lipid membranes and lipid-rich organelles are targets of peroxynitrite (ONOO-), a highly reactive species generated under nitrative stress. We report a membrane-localized phospholipid (DPPC-TC-ONOO-) that allows the detection of ONOO- in diverse lipid environments biomimetic vesicles, mammalian cell compartments, and within the lung lining. DPPC-TC-ONOO- and POPC self-assemble to membrane vesicles that fluorogenically and selectively respond to ONOO-. DPPC-TC-ONOO-, delivered through lipid nanoparticles, allowed for ONOO- detection in the endoplasmic reticulum upon cytokine-induced nitrative stress in live mammalian cells. It also responded to ONOO- within lung tissue murine models upon acute lung injury. We observed nitrative stress around bronchioles in precision cut lung slices exposed to nitrogen mustard and in pulmonary macrophages following intratracheal bleomycin challenge. Results showed that DPPC-TC-ONOO- functions specifically toward iNOS, a key enzyme modulating nitrative stress, and offers significant advantages over its hydrophilic analog in terms of localization and signal generation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: IScience Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: IScience Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos